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Effect of Active Ingredients After PP333 and CPPU on Physiological Characters of Rehmannia glutinosa f.Hueichingensis(Chan et Sehih)Hsiao Plantlet(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
147-151
Research Field:
Publishing date:

Info

Title:
Effect of Active Ingredients After PP333 and CPPU on Physiological Characters of Rehmannia glutinosa f.Hueichingensis(Chan et Sehih)Hsiao Plantlet
Author(s):
CHEN Mingxia1; 2; ZHANG Xiaoli1; 2; LI Mingjun1; 2; LI Jingjing1; 3; LI Xiangwu1
(1.College of Life Science,Henan Normal University,Xinxiang，Henan 453007；2.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,University of Henan Province,Xinxiang,Henan 453007；3.Pingdingshan Technology School,Pingdingshan,Henan 467091)
Keywords:
Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao; PP333; CPPU; physiological characters
PACS:
-
DOI:
10.11937/bfyy.201519036
Abstract:
The leaves of Rehmannia glutinosa f.Hueichingensis (Chan et Sehih) Hsiao were used as experimental material in this study.During the expansion period of microtuber，PP333,and CPPU were sprayed onto the plants at independent variables.The content of photosynthetic pigment，peroxidase (POD)，superoxide dismutase (SOD)，soluble sugar，and soluble protein were measured on in leaf samples collected on July，August，and September，changes of these physiological indexes were studied to lay the theoretical foundation for high-yield and high-quality production of Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao.The results showed that,the PP333 and CPPU treatment increased the content of total chlorophyll content and the chlorophyll a/b value in leaves but didn’t show obvious effect on the content of carotenoid.In the leaves of all treatments，the content of soluble sugar increased with the progress of plant growth.When the concentration of PP333 was 200 mg/L，the content of soluble sugar increased firstly and then decreased with the increase of CPPU concentration.In July，the content of soluble sugar reached the maximum value of 47.13 mg/g when the CPPU was at 0.667 mg/L；in August and September，the content of soluble sugar reached the maximum values of 61.61 mg/g and 110.26 mg/g，respectively，when the CPPU was at 1.333 mg/L.The content of soluble protein in the leaves increased firstly and then decreased with plant growth.In the same growth period，the soluble protein content in each treatment was higher than that in the control group.In July，the content of soluble protein showed a trend of increasing with the increase of the concentration of CPPU treatment，and reached the maximum value of 3.22 mg/g when CPPU was at 2.000 mg/L；in August and September，the soluble protein content showed a trend of firstly increased and then decreased with the increase of CPPU，when CPPU was at 1.333 mg/L，the soluble protein reached the maximum values of 6.49 and 6.53 mg/g，respectively.In all control and treatment groups，the POD and SOD activities showed a trend of firstly increasing and then decreasing.In the same growth period，both the SOD and POD activities were higher in treatment groups than control groups.With the increase of the CPPU treatment，the POD activity on July increased firstly and then decreased，and showed a maximum value of 27.50 U?g-1?min-1 when CPPU was at 1.333 mg/L；in August and September，the POD activity increased with the increase of CPPU treatment，and showed maximum values of,33.33 and 30.83 U?g-1?min-1，respectively.The SOD activities increased with the increase of CPPU treatment，and the maximum value was 138.86，148.00 and 138.10 U/g，respectively，in July，August and September.In summary，the combination of PP333 and CPPU treatment improved a series of physiological and biochemical indexes in Rehmannia glutinosa f.hueichingensis (Chan et Sehih) Hsiao，such as the photosynthetic pigment content，the POD and SOD activities，the contents of soluble sugar，and the soluble protein content.

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Last Update: 2015-11-06