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Sugar and Starch Metabolism and the Key-enzyme Gene Expression in Postharvest ‘Gala’ Apple Fruit(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
126-131
Research Field:
Publishing date:

Info

Title:
Sugar and Starch Metabolism and the Key-enzyme Gene Expression in Postharvest ‘Gala’ Apple Fruit
Author(s):
WEI Jianmei1; QI Xiudong2; YAN Fangjiao3
(1.Department of Ecology，Environmental Management College of China，Qinhuangdao，Hebei 066004；2.School of Continuing Education，Hebei Normal University of Science and Technology，Qinhuangdao，Hebei 066004；3.Hebei Academy of Agricultural and Forestry Sciences，Shijiazhuang，Hebei 050051)
Keywords:
‘Gala’ apple; sugar and starch metabolism; fruit softening; gene expression
PACS:
-
DOI:
10.11937/bfyy.201519031
Abstract:
Taking ‘Gala’ apple fruits as the test materials，the changes in the contents of soluble sugars and starch were analyzed in order to study the relationship between carbohydrate metabolism and fruit softening，the activities of related enzymes，and the key-enzyme gene expression after being treated at low temperature and with 1-MCP and ethephone.The results showed that the starch contents decreased rapidly and it played the most significant roles at the early stage of storage，and accompanied by a rapid rise rate in AM activity and MdAM gene expression，and either the starch content and AM activity or their correlation with firmness were significantly regulated by ethylene factor and low temperature treatment，showing that starch degradation had close correlations with ‘Gala’ fruit softening.The changes of sucrose，glucose and fructose contents showed significant correlations with fruit softening，which significantly regulated by low temperature and ethylene factor treatment.However，only the SPS activity increased and had significantly correlation with firmness loss，accompanied with a fast rise rate in the expression of MdSPS gene.Also，these phenomena were significantly promoted by ethephon treatment and obviously inhibited by low temperature and 1-MCP treatments，and the correlation between SPS activity and fruit firmness were affected in the same way.So above results showed that SPS played an important role in ‘Gala’ fruit softening by involving in sucrose metabolism.

References:

 

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Last Update: 2015-11-05