|Table of Contents|

Isolation and Characterization of the ‘Gala’ Apple MYB12 Gene Promoter(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
106-111
Research Field:
Publishing date:

Info

Title:
Isolation and Characterization of the ‘Gala’ Apple MYB12 Gene Promoter
Author(s):
QI Yingwei1LEI Qin2TIAN Jianwen1REN Xiaolin2
(1.Agricultural College,Ningxia University,Yinchuan,Ningxia 750021;2.College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100)
Keywords:
appleMYB12promotertomatoGUS
PACS:
-
DOI:
10.11937/bfyy.201519026
Abstract:
MYB12 promoter (ProMYB12) fragment was cloned and identified from genomic DNA of ‘Gala’ apple using the method of PCR.The sequence of this cloned promoter was analyzed by using the online database of Plant CARE and PLACE.The plant expression vector pCAMBI0390-ProMYB12-GUS was constructed and transformed into Agrobacteria GV3101.Through Arobacterium-mediated transformation,the constructed binary vector was introduced into wild type Micro-Tom (Lycopersion esculentum cv.Micro-Tom)tomato fruits,leaves and flowers in order to study the expression properties of this cloned promoter.The results showed that the cloned fragment was 1 290 bp long.Database searching showed that this fragment contained the essential elements such as TATA-box and CAAT-box,besides,there were several elements that responded to abiotic stress and hormones as well as many light responsive elements.Moreover,silico analysis of this isolated promoter sequence revealed the presence of some tissue specific cis-elements.Histochemical staining showed that GUS gene was highly expressed in the transgenic tomato flowers and around the tomato seeds which indicated the tissue specific characterization of the cloned promoter.

References:

 

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Last Update: 2015-11-05