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Study on Bulbil Induction Callus Differentiation From Cluster Buds of Lilium sulphureum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
101-105
Research Field:
Publishing date:

Info

Title:
Study on Bulbil Induction Callus Differentiation From Cluster Buds of Lilium sulphureum
Author(s):
ZHANG Xiangyu; CHEN Jie; JI Yun; YAN Xianjin; WANG Caiyun; RUAN Peijun
（Bijie Institute of Traditional Chinese Medicine，Bijie，Guizhou 551700）
Keywords:
Lilium sulphureum;  bulbil; callus; differentiation; cluster buds
PACS:
-
DOI:
10.11937/bfyy.201519025
Abstract:
The Lilium sulphureum callus were used as material by adopting the method of plant tissue culture，the effect of different concentrations of TDZ，NAA，2，4-D，ZT on bulbil induction callus differentiation from cluster buds of Lilium sulphureum was studied.The results showed that MS+0.3 mg/L TDZ+0.5 mg/L NAA+1.0 mg/L 2,4-D+0.5 mg/L ZT was the optimum medium,callus appeared in the fifteenth days,induction rate was (98.67±2.31)% of callus,cluster buds differentiated in the thirty-second days,induction rate was (98.67±2.31)% of cluster buds,average budding was (17.67±0.58)%.

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Last Update: 2015-11-05