|Table of Contents|

Study on Pedicels and Leaves Callus Induction of Osmanthus fragrans(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
95-101
Research Field:
Publishing date:

Info

Title:
Study on Pedicels and Leaves Callus Induction of Osmanthus fragrans
Author(s):
HU Tiantian; WANG Yali; YANG Xiulian; WANG Lianggui
（College of Landscape Architecture，Nanjing Forestry University，Nanjing,Jiangsu 210037）
Keywords:
tissue culture; callus; Osmanthus fragrans; mediums
PACS:
-
DOI:
10.11937/bfyy.201519024
Abstract:
The young pedicles and leaves of Osmanthus fragrans were used as the explants in the study.Through the plant tissue culture techniques,the experiment studied the establishment of Osmanthus fragrans sterile system,pedicels callus induction in different media formulations of varieties of O.fragrans groups and the influences of plant growth regulators，sucrose concentration，dark processing time etc.to leaves callus induction of different varieties of O.fragrans groups. The results showed that the best way to sterilize pedicels was using 5% NaClO for 3 min,the best way to sterilize leaves was using 0.1% HgCl for 5 min.The best mediums for pedicels of different varieties of O.fragrans were as follow,O.fragrans ’Boye Jingui’ was MS+2.00 mg/L 6-BA+0.40 mg/L 2,4-D，O.fragrans ’Zigeng Zi Yingui’ was B5+2.00 mg/L 6-BA+0.40 mg/L 2,4-D，O.fragrans ‘Yucheng Dangui’ was B5+1.00 mg/L 6-BA+0.10 mg/L 2,4-D，O.fragrans ’Rixiang’ was B5+2.00 mg/L 6-BA+0.10 mg/L 2,4-D.The results of leaves callus induction showed that,the best sucrose concentration was 25 g/L；it was best to put the mediums in dark condition for 22 days.The best medium for leaves of O.fragrans ‘Yucheng Dangui’ was MS+3.00 mg/L 6-BA+1.00 mg/L 2,4-D.The best medium for O.fragrans ‘Tianxiang Taige’ was MS+2.00 mg/L 6-BA+0.50 mg/L 2,4-D.

References:

 

[1]聂谷华，向其柏.桂花研究现状及其存在的问题[J].九江学院学报（哲学社会科学版），2008，26(3)：85-87.

[2]张丽霞，王毅彰.桂花的离体快繁技术[J].江苏农业科学,2013(3):42-43.

[3]蔡新玲,胡蕙露.桂花茎尖初代组织培养试验研究[J].安徽林业科技，2009 (1)：17-19.

[4]胡海波,黄丹,许岳香.木犀科植物组织培养研究综述[J].林业科技开发,2009(3):5-8.

[5]BONGA J M.树木组织培养[M].阙国宁,等译.北京:中国林业出版社，1988.

[6]潘瑞炽.植物组织培养[M].广州:广东高等教育出版社，2000：20-21.

[7]杜燕,蒋海玉,刘其宁.贮存过期油菜种子消毒方法的研究[J].种子,2003(2):39-40,42.

[8]WANG Y P，LIU Q C，LI A X，et al．In vitro selection and identification of drought tolerant mutants in sweet potato[J].Agricultural Sciences in China，2003，2(12)：1314-1320.

[9]董闪,李明,唐堃,等.白木香愈伤组织的诱导及培养[J].湖北农业科学,2014（15）:3673-3677.

[10]和凤美,朱永平,杨晓红,等.冬樱花愈伤组织诱导和抑制褐化初探[J].中国农学通报，2010(12)：130-134.

[11]宋会访,葛红,周媛,等.桂花离体培养与快速繁殖技术的初步研究[J].园艺学报,2005(4):738-740.

[12]喻晓雁,刘克旺,梁文斌.穗花杉组织培养初探[J].中南林学院学报,2005(2):55-58.

[13]任桂芳,王建红,冯慧,等.现代月季(Rosa hybrida)叶片植株再生体系的建立[J].园艺学报,2004(4):533-536.

[14]蔡新玲.不同桂花品种群组织培养的研究[D].合肥：安徽农业大学,2007.

[15]吕晋慧，孔冬梅.园艺植物组织培养[M].北京：中国农业科学技术出版社,2008:24.

[16]彭尽晖,吕长平,周晨.四季桂愈伤组织诱导与继代培养[J].湖南农业大学学报(自然科学版)，2003(2)：131-133.

[17]王文房,李修岭.樱花花柄的组织培养[J].安徽农业科学,2006(22):5839-5841.

[18]王金刚,张兴.园林植物组织培养技术[M].北京：中国农业科学技术出版社，2008:27-28.

[19]丁路明,龙瑞军,朱铁霞.2,4-D和6-BA对早熟禾愈伤组织诱导的影响[J].草原与草坪,2003(1):34-37.

[20]周宜君,周生闯,刘玉,等.植物生长调节剂对植物愈伤组织的诱导与分化的影响[J].中央民族大学学报(自然科学版),2007（1）:23-28.

[21]宋会访.桂花组织培养技术体系的研究[D].武汉：华中农业大学,2004.

Memo

Memo:
-
Last Update: 2015-11-04