|Table of Contents|

Plantlet Culture in Bioreactor and the Changes of Leave Stomata During Transplanting of Grape(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
26-29
Research Field:
Publishing date:

Info

Title:
Plantlet Culture in Bioreactor and the Changes of Leave Stomata During Transplanting of Grape
Author(s):
XIU Jingrun2; DAI Yue2; PIAO Xuanchun1; LI Meilan2; JIN Meiyu1
(1.Agriculture College，Yanbian University，Yanji，Jilin 133002；2.Yanbian Academy of Agricultural Sciences，Longjing，Jilin 133400)
Keywords:
grape; bioreactor; transportation; stomata
PACS:
-
DOI:
10.11937/bfyy.201519007
Abstract:
In order to explore a simple and low cost method for large scale production of plantlets in grape，with grape tissue cultured stem as explant，used air-lift bioreactor with raft method to study effect of sucrose concentrations on plantlet growth.Meanwhile，survival rate and stoma were observed after transplanting.The results showed that 20 g/L sucrose promoted plantlet growth，the plantlets with more numbers of node，deep green leaves，and high shoot and root biomass were obtained.The plantlets harvested from bioreactors were transplanted into substrates containing different amount of vermiculite and perlite，vermiculite was favorable for plantlet survive，all plantlets survived in the substrate which contained sole vermiculite.The close function in several stomata began from 10 days after transplanted，and the most of stomata were closed after 14 days.Therefore，the plantlets from bioreactors required moisture from beginning to 10 days after transplanted，and regular management was needed after 14 days after transplanted.

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Last Update: 2015-11-03