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Effect of High Temperature Stress on Physiological Characteristics and POD，SOD Isoenzyme in Brassica oleracea Seedlings(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年19
Page:
6-10
Research Field:
Publishing date:

Info

Title:
Effect of High Temperature Stress on Physiological Characteristics and POD，SOD Isoenzyme in Brassica oleracea Seedlings
Author(s):
LI Dahong1; ZHAO Li2; LI Zhen3; SUN Xiaoru3
(1.Department of Bioengineering，Huanghuai University，Zhumadian，Henan 463000；2.Garden Center of Huanghuai University，Zhumadian，Henan 463000;3.Garden of Zhumadian Bureau，Zhumadian，Henan 463000)
Keywords:
Brassica oleracea; high temperature stress; physiological characteristics; isoenzyme
PACS:
-
DOI:
10.11937/bfyy.201519002
Abstract:
Four different regions of the kales(Brassica oleracea）seedlings were used as materials，treated at (39±0.5)℃/(28±0.5)℃ (day/night temperature) for 8 days，and several related physiological indexes and SOD，POD isoenzyme in kale seedlings were determined，in order to discuss their plant adaptability under high temperature conditions.The results showed that the relative electric conductivity content，contents of malondialdehyde(MDA)，and free proline were gradually increased，but chlorophyll content decreased with the time of high temperature continued in different varieties.During the early 2 day after treatment，superoxide dismutase(SOD) and peroxidase(POD) activities increased dramatically，while fourth days later，they began to decline.But there were significant differences among different varieties.Research data showed that ‘Nagoya’ and ‘Jingguan red 2’ had stronger heat resistance than the ‘Red gull’ and ‘Leaf peony’.According to the analysis of POD，SOD isoenzyme bands，the band of SOD isoenzyme in ‘Nagoya’ and ‘Leaf peony’ were similar，and ‘Red gull’ and ‘Jingguan red 2’ were different.The bands of POD isoenzyme were similar between ‘Nagoya’ and ‘Jingguan red 2’，while ‘Leaf peony’ and ‘Red gull’ POD bands were similar，the bands of POD isoenzyme were difference between ‘Nagoya’，‘Jingguan red 2’ and ‘Leaf peony’，‘Red gull’.

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Last Update: 2015-10-29