|Table of Contents|

In vitro Conservation Technique of Illicium difengpi Germplasm(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年18
Page:
168-171
Research Field:
Publishing date:

Info

Title:
In vitro Conservation Technique of Illicium difengpi Germplasm
Author(s):
ZHANG Le1; 2;  LI Linxuan2; WEI Kunhua2; LYU Huizhen2; LI Minhui1; 2
（1.Baotou Medical College，Baotou，Inner Mongolia 014060;2.Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement，Guangxi Botanical Garden of Medicinal Plant，Nanning，Guangxi 530023）
Keywords:
rare or endangered; Illicium difengpi; germplasm resource; in vitro conservation
PACS:
-
DOI:
10.11937/bfyy.201518045
Abstract:
Taking Illicium difengpi as test material，to explore a method for the germplasm conservation in vitro of Illicium difengpi，the single buds were used as experimental materials to study the effects of inorganic salts，phytohormone and osmotic pressure，and the growth recovery was evaluated after in vitro conservation.The results showed that the best conservation condition was 1/2 MS basal medium containing 50 g/L sucrose，4.0 g/L agar powder and 5 g/L mannite supplemented with 1.0 mg/L CCC，in illuminated chamber,12~14 hours light/12~10 hours dark photoperiod of 2 000 lx light intensity at 25±2℃，the survival rate was 50% after ten month conservation，and most of them could growth well on propagation medium.

References:

 

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Memo

Memo:
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Last Update: 2015-10-23