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Identification of Bacteria Plasmid Type Collected From Crown Gall and Efficacy of Bacillus subtilis to Their Control in Pyrus betulaefolia Bge(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年18
Page:
129-133
Research Field:
Publishing date:

Info

Title:
Identification of Bacteria Plasmid Type Collected From Crown Gall and Efficacy of Bacillus subtilis to Their Control in Pyrus betulaefolia Bge
Author(s):
JIAO YanjingXING RuixiaoDU GuoqiangSHI XiaoxinZHANG Yuxing
(College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001)
Keywords:
Pyrus betulaefolia Bgecrown gallPCR assaypathogenicityBacillus subtilis
PACS:
-
DOI:
10.11937/bfyy.201518034
Abstract:
The plants of Pyrus betulaefolia Bge that affected by crown gall pathogen were applied in this experiment.Molecular biology methods were used to identify the plasmid of pathogen of P.betulaefolia Bge crown gall.The results showed that the bacteria sampled from the crown gall that grew on the roots of the one-year-old P.betulaefolia plants were cultured and purified.Four bacterium strains similar to Agrobacterium were obtained.PCR assay showed that four bacterium strains had specific band and could be classified to the type of nopaline.These strains caused crown gall after inoculated into the stems of the sunflower seedlings as index plants.The oxford cup method and the index plants inoculated with both Bacillus subtilis and the bacteria of crown gall were used to study the effect of Bacillus subtilis against the pathogen of P.betulaefolia Bge.The results showed that nine Bacillus subtilis strains showed inhibition to the Agrobacterium strains in in vitro test.Inoculating of the supernatant of Bacillus subtilis strains 9076 and 9161 showed completely resistance to the growth of the crown gall in the index plants..

References:

 

[1]王慧敏.植物根癌病的发生特点与防治对策[J].世界农业,2000(7)28-30.

[2]游积峰,谢学梅,陈培民,等.放射土壤杆菌HLB-2菌株防治葡萄根癌病的研究[J].生物防治通报,19906(1)35-37.

[3]WEBSTER JTHOMSON J.Genetic analysis of an Agrobacterium tumefaciens strain producing an agrocin active against biotype 3 pathogens[J].Molecular and General Genetics1988214142-147.

[4]梁志宏,王慧敏,王建辉.E26防治植物根癌病的效果及其稳定性初步研究[J].中国农业大学学报,20016(1)91-95.

[5]张静娟,那淑敏,余茂效,等.北京郊区根癌土壤杆菌生物型及质粒类型的鉴定[J].微生物学报,198424(4)369-375.

[6]张洁,师校欣,焦延静,等.葡萄根癌病病原菌类型鉴定及几种杀菌剂和抗菌素对其抑菌效果[J].河北农业大学学报,201336(3)77-81.

[7]张丽丽,常有宏,丁芳兵,等.2株枯草芽孢杆菌对梨轮纹病的室内抑制作用研究[J].果树学报,201027(5)823-827.

[8]李社增,鹿秀云,马平,等.防治棉花黄萎病的生防细菌NCD-2的田间效果评价及其鉴定[J].植物病理学报,200535(5)451-455.

[9]刘慧芹,韩巨才,赵廷昌,等.果树内生拮抗细菌的筛选鉴定及其生防作用研究[J].园艺学报,201441(2)335-342.

[10]秦瑶,王苇,郭秉娇,等.2株枯草芽孢杆菌对大肠杆菌和沙门氏菌的体外抑菌试验研究[J].中国畜牧兽医,201441(1)207-210.

[11]HAMMAMI IRHOUMA AJAOUADI Bet al.Optimization and biochemical characterization of a bacteriocin from a newly isolated Bacillus subtilis strain 14B for biocontrol of Agrobacterium spp.strains[J].The Society for Applied Microbiology200948253-260.

[12]陈凡.水生拉恩氏菌HX2菌株防治葡萄根癌病的初步研究[D].北京:中国农业大学,2007.

[13]TAN B SYABUKI JMATSUMOTO Set al.PCR primers for identification of opine types of Agrobacterium tumefaciens in Japan[J].Journal of General Plant Pathol200369258-266.

[14]FILO ASABBATINI PSUNDIN G Wet al.Grapevine crown gall suppression using biological control and genetic engineeringa review of recent research[J].American Journal of Enology and Viticulture2013641-14.

[15]赵新林,赵思峰.枯草芽孢杆菌对植物病害生物防治的作用机理[J].湖北农业科学,201150(15)3025-3028.

[16]但红侠,董宁,万素梅,等.枯草芽孢杆菌对棉花枯、黄萎病的抑制作用研究[J].新疆农业科学,201047(11)2221-2225.

[17]何红.辣椒内生枯草芽孢杆菌(Bacillus subtilis)防病促生作用的研究[D].福州:福建农林大学,2003.

[18]高晓岗.生防细菌菌株GXG-2-2的筛选、鉴定及其产生的抑菌活性物质的初步研究[D].杨凌:西北农林科技大学,2007.

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Last Update: 2015-10-23