|Table of Contents|

Construction of ERECTA Gene Plant Expression Vector that Based on In-fusion Technology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年18
Page:
110-115
Research Field:
Publishing date:

Info

Title:
Construction of ERECTA Gene Plant Expression Vector that Based on In-fusion Technology
Author(s):
CHEN FeifanWANG QiqiZHONG HuiliFU BingbingREN DanliLI Yuhong
(Horticulture College,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100)
Keywords:
cucumberERECTAlong-segment amplificationIn-fusionexpression vector
PACS:
-
DOI:
10.11937/bfyy.201518029
Abstract:
Taking cucumber breed of ‘Changchun Mici’ (CCMC) as material,using high fidelity polymerase,Iproof and In-fusion technology that not based on digestion to construct plant expression vector of ERECTA gene that was driven by its own promoter for discussing the function of ERECTA gene and providing technology to improve resistance inheritance in cucumber.The results showed that two segments amplified from ‘CCMC’ genome DNA were named CsgERECTA--Flag which was 8 940 bp and CsgERECTA-Poly which was 9 812 bp.Plasmid PCR,gradient segments PCR and sequencing were conducted,which showed that two pCambia3301-gERECTA plant expression vector were both constructed and transformed into Agrobacterium tumefaciens EHA105.

References:

 

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Last Update: 2015-10-23