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Research of Tissue Culture and Regenerated of the Different Explants of Chrysanthemum indicum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年18
Page:
106-109
Research Field:
Publishing date:

Info

Title:
Research of Tissue Culture and Regenerated of the Different Explants of Chrysanthemum indicum L.
Author(s):
WANG Zibu; MO Guoxiu; LUO Huilan; ZHANG Deying
(College of Chemistry and Life Science，Guizhou Normal College，Guiyang,Guizhou 550018)
Keywords:
Chrysanthemum indicum L.; explants; tissue culture; regeneration
PACS:
-
DOI:
10.11937/bfyy.201518028
Abstract:
Chrysanthemum indicum L.was used as explants to study rapid propagation through tissue culture.The effect of media components on the induction of shoot regeneration for different types of explants in five genotypes of Chrysanthemum indicum L.were investigated.The results showed that forming the callus for different genotypes explants cultured on media containing suitable 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BA) concentrations was easy，but shoot regeneration from the callus proved difficult.The frequency of shoot regeneration from chrysanthemum’s different types of explants for all the genotypes were in turn as follows：flour bud>petal>stem>leaf.The best culture medium for the chrysanthemum’s explants was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L.The ability of inducing shoot regeneration for different explants of chrysanthemum was very different，and the regenerated frequency of flour bud and petal was the highest.Shooting culture was on 1/2 MS medium supplemented with NAA 0.2 mg/L.These results indicate that the experiment successfully established the plant regeneration system of Chrysanthemum indicum L.，and successfully obtained the regenerated plant.

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Last Update: 2015-10-23