|Table of Contents|

Analysis of Genetic Diversity of Wild Lepista nuda Population From Zhaotong，Yunnan(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年17
Page:
132-136
Research Field:
Publishing date:

Info

Title:
Analysis of Genetic Diversity of Wild Lepista nuda Population From Zhaotong，Yunnan
Author(s):
WEI Hailian; LIU Zhixi; ZHANG Wanjie; HE De; LI Cuixin; MA Jian
(College of Life Science，Southwest Forestry University,Kunming,Yunnan 650000)
Keywords:
Lepista nuda; internal transcribed spacers (ITS); single nucleotide polymorphism (SNPs); genetic diversity
PACS:
-
DOI:
10.11937/bfyy.201517035
Abstract:
Lepista nuda from Zhaotong,Yunnan Province was used as the research object，its ITS sequence and single nucleotide polymorphism (SNP) were analyzed.The results showed that：combined with morphological and molecular identification，the collected wild mushrooms were confirmed as Lepista nuda；66 strains L.nuda’s ITS sequence length was 666-671 bp，ITS1:241-244 bp，5.8S:155 bp，ITS2:267-275 bp；G+C content was 41.5%-42.4%；the average genetic distance of all samples was 0.003 0.By sequence alignment，seven SNPs loci were screened out：three singleton variable sites，four parsimony informative sites；transition and transversion ratio was 0.672.The nucleic acid polymorphisms index Pi of the population was 0.002 91.Each site nucleotide polymorphisms index Eta was 0.002 36.Neighbor (neighbour-joining，NJ) phylogenetic tree was constructed.Bootstrap support rate was low，which indicated that genetic distance of each strain in the population was very close and their genetic diversity was very low.In summary，the genetic diversity of the wild Lepista nuda population from Zhaotong was very low so that its protection should be strengthened.

References:

 

[1]陈士俞.食用菌生产大全[M].北京:中国农业出版社,1988.

[2]谢支锡,王云,王柏,等.长白山伞菌图志[M].长春:吉林科学技术出版社,1986.

[3]陈士瑜,陈惠.菇菌栽培手册[M].北京:科学技术文献出版社,2003.

[4]YANG S,HAN J Y,JOO E Y,et al.Study on the antitumor effects of extmcts from Lepista nuda mushroom[J].Journal of the Korean Society of Food Science and Nutritio,2005,3(34)：317-322.

[5]KIM S D,KIM J H,KIM J B,et al.Mycelial culture conditions of Lepista nuda and extracellular enzyme activity[J].The Korean Journal of Microbiology,2005,3(6):42.

[6]史全良,赵卫国.桑树ITS序列测定及特点的初步分析[J].蚕业科学，2001,27(2):140-141.

[7]FRUDAKIS T,VENKATE S K.A classier for the SNP-based inference of ancestry[J].Journal of Forensic Sciences,2003,48(4)：771-782.

[8]GAO Z X,YAN L,WANG X F,et al.Sodium nitroprusside (SNP) alleviates the oxidative stress induced by NaHCO3 and protects chloroplast from damage in cucumber[J].African Journal of Biotechnology,2012,11(27):6974-6982.

[9]吴发红,黄东益,黄小龙,等.几种真菌DNA提取方法的比较[J].中国农学通报,2009,25(8):62-64.

[10]HALL T A.BioEdit:a user friendly biological sequence alignment editor and analysis program for windows 95/98/NT[J].Nucleic Acids Symposium Series,1999,41:95-98.

[11]WANG L,ROOSINCK M J.Comparative analysis of expressed sequences reveals a conserved pattern of optimal codon usage in plants[J].Plant Molecular Biology,2006,61(4-5):699-710.

[12]LARKIN M A,BLACKSHIELDS G,BRONW N P,et al.Clustal W and clustal X version 2.0[J].Bioinformatics,2007,23(21):2947-2948.

[13]KUMAR S,NEI M,DUDLEY J,et al.MEGA:a biologist-centric software for evolutionary analysis of DNA and protein sequences[J].Briefbioinform,2008,9(4):299-306.

[14]LIBRADA P,ROZAS J.DnaSP v5：A Software for comprehensive analysis of DNA polymorphism data[J].Bioinformatics,2009,25(11):1451-1452.

[15]PLOTREE D,PIOTGRAM D.Phylip-phylogeny inference package (version 3.2)[J].J Felsenstein Cladistics,1989,5(1):164-166.

[16]LANDEWEERT R,LEEFLANG P,KUYPER T W,et al.Molecular identification of ectomycorrhizalm mycelium in soil horizons[J].Appliedand Environmental Microbiology,2003,69(1):327-333.

[17]谢富刚,刘雁英,邱慧兰.紫丁香蘑菌丝体生物学特性研究初报[J].现代农业科技,2010(2):72-76.

[18]高锦明,董泽军.紫丁香蘑的化学成分[J].中草药,2002,33(5):398-401.

[19]周峰,王瑞娟,李玉,等.珍稀食药用菌紫丁香蘑的研究进展[J].食用菌学报,2010,17(4):79-83.

[20]赵婷.紫丁香蘑菌丝体培养特性及人工驯化栽培研究[D].延边:延边大学,2010.

[21]李萌伟,李芳菲,樊志宏,等.紫丁香蘑 ISSR-PCR反应体系的正交优化与建立[J].生物技术,2013,23(6):55-59.

[22]LEE Y S,KIM N W,KIM J B.Genetic variation based on random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) region sequences in Lepista nuda[J].Journal of the Korean Society of Food Science and Nutrition,2012,22(11):1470-1476.

Memo

Memo:
-
Last Update: 2015-10-10