|Table of Contents|

Expression Analysis of Apple Chitinase Gene Induced by Marssonina mali in Germplasms With Different Resistances(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年17
Page:
104-107
Research Field:
Publishing date:

Info

Title:
Expression Analysis of Apple Chitinase Gene Induced by Marssonina mali in Germplasms With Different Resistances
Author(s):
ZHANG WeiTIAN YikeKONG XianghuaHOU DongliangWANG Caihong
Keywords:
(College of HorticultureQingdao Agricultural UniversityQingdaoShandong 266109)
PACS:
-
DOI:
10.11937/bfyy.201517027
Abstract:
Plant chitinase,one of the important pathogenesis-related (PR) proteins in plants,has the effect of resistance to fungal diseases by degradating the cell walls of fungi.In this study,leaves of apple cultivar ‘Fuji’,‘Qinguan’ and one selection derived from their hybirdization ‘QF-2’ were used as materials.With quantitative real-time PCR method,expression of apple chitinase gene MdChi in response to marssonina blotch was analysed.The results showed that MdChi gene was expressed in all these three germplasms.The expression quantity in the susceptible cultivar ‘Fuji’ was significantly higher than the resistant cultivar ‘Qinguan’ and their hybrid ‘FQ-2’.Twenty-four hours after the inoculation,the expression level in ‘Fuji’ leaves increased obviously,and then sharply declined.However,the expression of this gene maintained at low levels and there was no significant change in both of the resistant cultivar ‘Qinguan’ and the resistant hybird ‘FQ-2’ during 96 hours after the inoculation.

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Last Update: 2015-10-10