|Table of Contents|

Comparative Analysis and Molecular Identification of matK Gene From Spatholobus suberectus and Its Adulterants(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年17
Page:
94-98
Research Field:
Publishing date:

Info

Title:
Comparative Analysis and Molecular Identification of matK Gene From Spatholobus suberectus and Its Adulterants
Author(s):
HUANG Qionglin1; MA Xinye2; 3; ZHAN Ruoting2; 3; CHEN Weiwen2; 3
(1.Guangdong Medical University，Zhanjiang,Guangdong 524023;2.Research Center of Chinese Medicinal Resource Science and Engineering，Guangzhou University of Chinese Medicine，Guangzhou,Guangdong 510006;3.Key Laboratory of Chinese Medicinal Resource from Lingnan，Ministry of Education，Guangzhou,Guangdong 510006)
Keywords:
Spatholobus suberectus Dunn.; matK gene; sequence analysis; molecular identification; adulterants
PACS:
-
DOI:
10.11937/bfyy.201517024
Abstract:
In order to investigate the efficiency of matK gene on identifying Spatholobus suberectus and its adulterants，S.suberectus and four common adulterants were used as materials in this study.Genomic DNA was isolated from fresh leaves and dry materials of S.suberectus，and matK gene was amplified and sequenced using a pair of universal primers.matK sequences of the adulterants were downloaded from GenBank database.The sequences were aligned and analyzed to find the difference and then genetic distance were calculated and the clustering tree was constructed using biological software.The results revealed that matK sequences acquired from S.suberectus and its adulterants contained 690 base pairs with an average GC content of 31.1%．The maximal intraspecific distances among S.suberectus were 0.001，whereas the interspecific distance between S.suberectus and its adulterants ranged from 0.114 to 0.288.The clustering tree based on matK sequences can intuitively discriminate S.suberectus and its adulterants.matK gene can be used as a standard DNA sequence to distinguish Spatholobus suberectus and its adulterants.

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Last Update: 2015-10-10