|Table of Contents|

Establishment of High Performance Liquid Chromatography Method forDetermination of Procyanidin From Musalais Wine in Xinjiang(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年16
Page:
126-130
Research Field:
Publishing date:

Info

Title:
Establishment of High Performance Liquid Chromatography Method forDetermination of Procyanidin From Musalais Wine in Xinjiang
Author(s):
WANG Weihua1; CAO Yuan2; MO Juntao1; TIAN Muxing1; LIU Fei1
(1.College of Life Science，Tarim University,Xinjiang Production and Construction Corps Key Laboratory of Deep Processing of Agricultural Products in South Xinjiang，Alar,Xinjiang 843300；2.Alar Quality and Technical Supervision Bureau，Alar,Xinjiang 843300)
Keywords:
high performance liquid chromatography (HPLC); Musalais wine; procyanidins; recovery
PACS:
-
DOI:
10.11937/bfyy.201516030
Abstract:
Taking cyanidin,catechin,epicatechin and epicatechin-3-gallate as standards，a high performance liquid chromatograph (HPLC) method for determination of procyanidin content from Musalais was established using SHIMADZU LC-20A HPLC and diode array detector.The results showed that optimized chromatographic condition was as follow：column with Inertsil DOS-3，5 μm 4.6 mm×250 mm;mobile phase A was methanol，and mobile phase B was ultrapure water;proportion of mobile phase:mobile phase A was 35%，and mobile phase B was 65%;flow rate was 1 mL/min;sample volume was 10 μL;column temperature was 38℃.Using this HPLC method to detect Musalais, peak effect was well，standard curve was normal，and recoveries were 82% to 93%.This method had the advantages of simple operation,high accuracy，and short detection time.So it was successfully applied to determine procyanidins form Musalais.

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Last Update: 2015-09-02