|Table of Contents|

Molecular and Morphological Identification of Peony Root Rot Pathogen(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年16
Page:
116-119
Research Field:
Publishing date:

Info

Title:
Molecular and Morphological Identification of Peony Root Rot Pathogen
Author(s):
CHENG Yumei1; ZHAO Dan2; KANG Yebin3
(1.School of Chemical and Pharmaceutical，Henan University of Science and Technology，Luoyang,Henan 471003；2.Huarui College,Xinyang Normal University，Xinyang,Henan 464000；3.School of Forestry，Henan University of Science and Technology，Luoyang,Henan 471003)
Keywords:
peony; F.solani; morphological identification; molecular identification
PACS:
-
DOI:
10.11937/bfyy.201516027
Abstract:
Taking the typical infected plants of peony root rot as test materials，which from in mainly peony garden in Luoyang，Henan Province.The pathogens were isolated by tissue separation，tested according to the standard Koch’s postulation methods，and identified by the methods of microscopy and molecules，the pathogen species of peony root were studied.The results showed that four strains all produced thick，loose cotton wool hypha and yellow，red，violet colors in PDA.Macroconidium were falciform or ellipse，colourless，multi-cell；microconidia were oval to ellipse，colourless，one-celled or bicellular.those strains of Fusarium could infect in vitro peony root and make it black.The DNA sequence homology of F1 to F4 strains and Nos in GenBank sequence database of HM214456.1 and AB498917.1 F.solani was 99%-100%.Accordingly，the pathogen of peony root rot disease was identified as F.solani.

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Last Update: 2015-09-02