Study on the One-step Culture of Rapid Propagation Technology in Euryale ferox(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the One-step Culture of Rapid Propagation Technology in Euryale ferox

Author(s):: LI Yanlian1; ZHANG Huali2; ZHAO Qiang1; WEI Xiaoli1; WANG Yanxin1; CHEN Jie1; ZHANG Xicai1; (1.Life Science and Engineering Department,Jining University，Qufu，Shandong 273155；2.School of Pharmacy，Jining Medical University，Rizhao,Shandong 276826)

Keywords:: Euryale ferox; one-step culture; rapid propagation technology; tissue culture

Abstract:: Taking Euryale ferox leaves and stem segments as explants，MS as culture medium，the additional 2，4-D or NAA and 6-BA or KT，according to the principle of orthogonal design，using factor analysis and cluster analysis， the effect of different ratio of hormone on rapid propagation of Gordon Euryale step into seedlings was studied.The results showed that the best hormone matchings was MS+NAA 0.2 mg/L+6-BA 2.0 mg/L，others were MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L,MS+2,4-D 0.2 mg/L+6-BA 1.0 mg/L,MS+2,4-D 1.0 mg/L+KT 1.0 mg/L,MS+NAA 1.5 mg/L+6-BA 1.0 mg/L.

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Last Update: 2015-09-02