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Establishment of a Novel Micropropagation System Using Garlic Slice as the Explants(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年16
Page:
101-106
Research Field:
Publishing date:

Info

Title:
Establishment of a Novel Micropropagation System Using Garlic Slice as the Explants
Author(s):
REN Chunxue; WANG Zhen; FAN Baoli; LIU Xiaoying; WANG Zhenying; PENG Yongkang
(College of Life Science，Tianjin Normal University，Tianjin 300387)
Keywords:
garlic micropropagation system; callus; strong buds culture
PACS:
-
DOI:
10.11937/bfyy.201516024
Abstract:
Developed a novel micropropagation technology system using garlic slice as the explants by the plant tissue culture technology.The results showed that the callus induction and subculture medium were the same as MS+1.5 mg/L 2,4-D+0.5 mg/L KT；the differentiation culture medium was MS+5 mg/L 6-BA+1.0 mg/L NAA；the strong buds culture medium was MS+1.0 mg/L KT+1.0 mg/L GA+0.2 mg/L NAA+2.0 mg/L 6-BA and the rooting culture medium were the basis MS.The plantlets was trained through indoor and field for two times and transplanted to soil later under the plastic film before winter.In the T0 generation，we harvested more than 94% garlic which had two or more cloves.The technical system provided an important safeguard for the propagation of virus-free garlic.

References:

 

[1]HAVRANEK P，NOVAK F J.The bud formation in the callus cultures of Allium sativum L.[J].Pflanzenphysiol，1973，68:308-318.

[2]杨乃博.大蒜全展叶愈伤组织的诱导和植株再生[J].植物生理学通讯，1981，5(6)：47-48.

[3]唐巧玲,王旭静,王志兴.以根尖为外植体建立大蒜的组织培养体系[J].生物技术进展,2011,1（2）：140-145.

[4]TUBI〖KG-*3〗C〖DD(-*2/3〗′〖DD)〗 L，ANA〖KG-*3〗C〖DD(-*2/3〗ˇ〖DD)〗KOV C，MILOJEVI〖KG-*3〗C〖DD(-*2/3〗′〖DD)〗 J，et al.High variability in the tissue culture response of root-tips of Allium ascalonicum individuals and optimization of the regeneration procedure[J].Plant Cell Tiss Organ Cult,2014，118:101-110.

[5]陈世儒,黄菊辉.大蒜离体快繁及脱毒[J].园艺学报,1991,3(3):133-137.

[6]汪盛松,邓晓辉,邱正明,等.兴山紫皮蒜的组培快繁技术研究[J].湖北农业科学,2013,52(7):1689-1691.

[7]张昌伟,候喜林,袁建玉,等.太仓大蒜根尖离体培养直接诱导不定芽及试管鳞茎的形成[J].植物生理学通讯,2004,40（2）：167-170.

[8]LUCIANI G F,MARY A K,PELLEGRINI C,et al.Effects of explants and growth regulators in garlic callus formation and plant regeneration[J].Plant Cell Tiss Organ Cult,2006,87:139-143.

[9]张素芝,李继蓉.秋水仙素诱导大蒜四倍体的研究[J].核农学报,2006,20(4):303-308.

[10]徐培文，刘冰江，孔素萍,等.大蒜种质创新和育种研究进展[J].中国蔬菜,2006(6):31-33.

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Last Update: 2015-09-02