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Study on Tissue Culture and Mycorrhizal Fungi of Cymbidium lianpan(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年15
Page:
111-113
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture and Mycorrhizal Fungi of Cymbidium lianpan
Author(s):
DUAN Xuetian1; CHEN Yuwei1; WANG Fang2; LI Tingting1; WU Jianrong1; 2
(1.Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming,Yunnan 650224；2.Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions, College of Forestry, Southwest Forestry University, Kunming,Yunnan 650224)
Keywords:
Cymbidium lianpan;  tissue culture; mycorrhizal fungi;  symbiosis; mycorrhizal
PACS:
-
DOI:
10.11937/bfyy.201515029
Abstract:
Using wild Cymbidium lianpan and Cymbidium seed as test materials, using the method of isolating and identificating endophytic fungus, examining the interaction between aseptic seedlings and endophytic fungus in Cymbidium lianpan and making paraffin section of the mycorrhizal structure observation,to study the effect of mycorrhiza fungi on cymbidium lianpan growth,and find out the suitable mycorrhizal fungi for growth of Cymbidium. The results showed that from Baoshan field collection 12 cymbidium lianpan of 10 strains of isolated Cymbidium, there were three suits with the somaclone symbiotic cultivation, respectively was Rhizoctonia sp., Chaetomium sp., Fusarium sp., among them,the effect of Rhizoctonia sp. was the best, had a good role in promoting growth of sterile somaclone hypha and sterile somaclone in the root formation.

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Last Update: 2015-08-20