|Table of Contents|

Tissue Culture and Plantlet Regeneration of Ipomoea pes-caprae(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年15
Page:
104-106
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Plantlet Regeneration of Ipomoea pes-caprae
Author(s):
LI Haili; TAN Feili; LIU Kaidong; CHENG Xialan
(School of Life Science and Technology，Lingnan Normal University，Zhanjiang，Guangdong 524048)
Keywords:
Ipomoea pes-caprae; tissue culture; plantlet regeneration
PACS:
-
DOI:
10.11937/bfyy.201515027
Abstract:
Taking different explants of Ipomoea pes-caprae as material,using MS media with different combination of 6-BA,NAA concentration,the effect of 6-BA,NAA on callus induction，bud initiation，shoot proliferation were studied to optimize the tissue culture of Ipomoea pes-caprae. The results showed that the initial medium for the callus induction was MS+6-BA 0.75 mg/L+NAA 0.25 mg/L，and the optimal explants were leaves.The best medium for adventitious bud differentiation was MS+6-BA 1.0 mg/L+NAA 0.1-0.5 mg/L.The best subculture media for shoot proliferation was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L.The rooting rate was 87.5% in 1/2MS medium.

References:

 

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Last Update: 2015-08-18