|Table of Contents|

Cloning and Expression Analysis of Allene Oxide Cyclase Gene (CfAOC) in Cymbidium faberi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年14
Page:
84-89
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Allene Oxide Cyclase Gene (CfAOC) in Cymbidium faberi
Author(s):
ZHOU Yin1WANG Songtai1DONG Kun1HONG Huazhu12GE Xiaoxia1
(1.School of Life Science and Biotechnology,Wuhan Bioengineering University,Wuhan,Hubei 430415;2.Institute of Entomology,Central China Normal University,Wuhan,Hubei 430079)
Keywords:
Cymbidium faberiAllene oxide cyclase gene (CfAOC)jasmonate biosynthesisexpression analysis
PACS:
-
DOI:
10.11937/bfyy.201514023
Abstract:
Taking Cymbidium faberi as material,focus on one of the most crucial enzymes,CfAOC,which participates in the biosynthesis of methyl jasmonate,the main component of the flower fragrance in C.faberi.The paper was to provide theoretical basis for the identification of molecular mechanism in the flower fragrance biosynthesis in C.faberi.The results showed the full-length and CDS sequences of CfAOC were obtained using PCR amplification with gDNA and cDNA of C.faberi as templates,respectively.The sequencing results demonstrated that CfAOC had 3 exons truncated with 2 introns.CfAOC was predicted to be located in chloroplast by subcellular localization,with a conserved amino acid region of allene oxide cyclase domain.The phylogenetic tree proved that CfAOC was homologous to AOC in the other plants as well,involved in the jasmonates biosynthesis.The spatial temporal specific expression analysis revealed that higher transcript levels of CfAOC were in flower at blooming stage,which suggested that CfAOC could be related with the flower scent biosynthesis in C.faberi.

References:

 

[1]黄暖爱,黄绵佳.兰科植物保育研究概况[J].亚热带植物科学,200736(4)72-76.

[2]马丽娅,孙晓琴,贾文杰,等.春兰ISSR-PCR反应体系的建立和优化[J].安徽农业科学,200836(34)14192-14194.

[3]THIRUVENGADAM MYANG C.Ectopic expression of two MADS box genes from orchid (Oncidium Gower Ramsey) and lily (Lilium longiflorum) alters flower transition and formation in Eustoma grandiflorum[J].Plant Cell Rep200928(10)1463-1473.

[4]CHANDLER S FBRUGLIERA F.Genetic modification in floriculture[J].Biotechnol Lett201133(2)207-214.

[5]THIRUVENGADAM MCHUNG IYANG C.Overexpression of Oncidium MADS box (OMADS1) gene promotes early flowering in transgenic orchid (Oncidium Gower Ramsey) [J].Acta Physiol Plant201234(4)1295-1302.

[6]HOSSAIN M,MUSHAROFKANT Ret al.The Application of Biotechnologyto Orchids[J].Crit Rev Plant Sci201332(2)69-139.

[7]OMATA ANAKAMURA SYOMOGIDA Ket al.Volatile components of TO-YO-RAN flowers (Cymbidium faberi and Cymbidium virescens)[J].Agric Biol Chem199054(4)1029-1033.

[8]彭红明.中国兰花挥发及特征花香成分研究[D].北京:中国林业科学研究院.2009.

[9]TURNER J GELLIS CDEVOTO A.The jasmonate signal pathway[J].Plant Cell200214153-164.

[10]PAUW B,MEMELINK J.Jasmonate-responsive gene expression[J].J Plant Growth Regul200523(3)200-210.

[11]ZHANG HMEMELINK J.Regulation of secondary metabolism by jasmonatehormones[M].Plant-derived Natural Products,2009181-194.

[12]HAUSE BSTENZEL IMIERSCH Oet al.Tissue-specific oxylipin signatureof tomato flowers-allene oxide cyclase is highly expressed in distinct flower organs and vascular bundles[J].Plant J200024(1)113-126.

[13]STENZEL IHAUSE BMAUCHER Het al.Allene oxide cyclase dependence of the wound response and vascular bundle-specific generation of jasmonates in tomato-amplification in wound signaling[J].Plant J2003a33(3)577-589.

[14]STENZEL IHAUSE BMIERSCH Oet al.Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana[J].Plant Mol Biol2003b51(6)895-911.

[15]BETTINA HIRENE SOTTO Met al.Occurrence of the allene oxide cyclase in different organs and tissues of Arabidopsis thaliana[J].Phytochemistry200364(5)971-980.

[16]IRENE SOTTO MCAROLIN Det al.ALLENE OXIDE CYCLASE (AOC) gene family members of Arabidopsis thalianatissue-and organ-specific promoter activities and in vivo heteromerization[J].J Exp Bot201263(17)6125-6138.

[17]HELMUT MIRENE SOTTO Met al.The allene oxide cyclase of barley (Hordeum vulgare L.)--cloning and organ-specific expression[J].Phytochemistry200465(7)801-811.

[18]蒋细旺,包满珠,李智崎,等.菊花DNA提纯方法的优化[J].江汉大学学报,200219(3)42-44.

[19]郝朝运,刘鹏,郭卫东,等.忍冬科部分植物DNA提取方法的研究[J].浙江师范大学学报(自然科学版),20062(1)92-98.

[20]CENZANO AABDALA GHAUSE B.Cytochemical immuno-localization of allene oxide cyclasea jasmonic acid biosynthetic enzymein developing potato stolons[J].J Plant Physiol2007164(11)1449-1456.

[21]RIEMANN MHAGA KSHIMIZU Tet al.Identification of rice Allene Oxide Cyclase mutants and the function of jasmonate for defence against Magnaportheoryzae[J].Plant J201374(2)226-238.

[22]ZHAO YDONG WZHANG Net al.A wheat allene oxide cyclase gene enhancessalinity tolerance via jasmonate signaling[J].Plant Physiol2014164(2)1068-1076.

[23]MIERSCH OWEICHERT HSTENZEL Iet al.Constitutive overexpression of allene oxide cyclase in tomato (Lycopersicon esculentum cv Lukullus) elevates levels of some jasmonates and octadecanoids in flower organs but not in leaves[J].Phytochemistry200465(7)847-856.

[24]KOMBRINK E.Chemical and genetic exploration of jasmonate biosynthesis and signaling paths[J].Planta2012236(5)1351-1366.

Memo

Memo:
-
Last Update: 2015-08-12