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Extraction and Determination of Total Flavonoids From Cordyceps militaris Clutrued by Different Cultural Medium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
137-139
Research Field:
Publishing date:

Info

Title:
Extraction and Determination of Total Flavonoids From Cordyceps militaris Clutrued by Different Cultural Medium
Author(s):
SUN Jingbo; YU Jing; AN Liping; JIA Hao; DU Peige
Pharmaceutical College,Beihua University，Jilin，Jilin 132013
Keywords:
Cordyceps militaris; total flavonoids; ultraviolet spectroscopy
PACS:
S 6;S 567.3+5
DOI:
10.11937/bfyy.201513039
Abstract:
Taking Cordyceps militaris from different sources as test materials，the extraction technology of total flavonoids in C.militaris was optimized by orthogonal test，the chromogenic agent and the determine wavelength selection depended on the shapes，positions and intensity of UV absorbance bands.The results showed that total flavonoids content in C.militaris was the highest when refluxed extracted with 40 times of 70% alcohol for 4 hours (90℃)，and then determined by UV at 265 nm after being treated with 0.1 mol/L AlCl3.The total flavonoids contents in C.militaris growing on rice medium，and in fruit bodies，whole grass，pupa body of C.militaris growing on silkworm pupa were 3.700 8 mg/g，4.039 8 mg/g，3.219 2 mg/g，2.502 4 mg/g，respectively.

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Last Update: 2015-08-21