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Study on Extracting DNA From Peach Leaves Efficiently(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
120-125
Research Field:
Publishing date:

Info

Title:
Study on Extracting DNA From Peach Leaves Efficiently
Author(s):
LIU Hangkong; WANG Anzhu; ZHAO Caiping; HAN Mingyu; LI Jinjin; LI Fang
College of Horticulture，Northwest Agriculture and Forestry University，Yangling,Shaanxi 712100
Keywords:
peach; leaf; high throughput; genome DNA
PACS:
S 662.103.6
DOI:
10.11937/bfyy.201513034
Abstract:
Taking different maturities of leaves from peach progenies population as material，commercial kit was experimented as control group.The effect of the parameters of retsch about the grinding bead sizes，the frequency and time of vibration on yield and quality of DNA were investigated by the method of high throughput extracting DNA.The results showed that adding 0.3% ascorbic acid，increasing the concentration of PVP to 4% and soaring the concentration of NaCl to 12% at the base of usual CTAB extraction buffer,the pigment and protein，polysaccharide were eliminated，and the problem appeared in the process of extracting DNA by the kits were effectively solved.Combining with the modified extraction method，the grinding pretreatment was screened.With the vibration frequency of 30 times/sec in 60 seconds by 5 mm grinding bead or the vibration frequency of 25 times/sec in 60 seconds by 5 mm grinding bead，a high throughput approach for DNA isolation from different maturity of leaves were acquired.It is not only considered to be a good ways to save time and dispense with much labor at extracting DNA，but also it was applied to extracting DNA from different maturity of peach leaves.More importantly，it could get high yield，reliable quality DNA for the genome sequencing.This study would provide guidance for technology of high performance DNA isolation.

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Last Update: 2015-08-21