|Table of Contents|

Comparative Study on Methods for Testing Pollen Viability of Camellia azalea(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
88-90
Research Field:
Publishing date:

Info

Title:
Comparative Study on Methods for Testing Pollen Viability of Camellia azalea
Author(s):
XU Bin1; PENG Lixia2; PAN Wen1; ZHANG Fangqiu1
1.Guangdong Academy of Forestry，Guangzhou,Guangdong 510520；2.Guangdong Ecoengineering Vocational College，Guangzhou,Guangdong 510520
Keywords:
Camellia azalea; pollen viability; pollen germination in vitro; coloration
PACS:
S 685.14
DOI:
10.11937/bfyy.201513026
Abstract:
Taking 4-year-old grafted Camellia azalea as test material,effects of different methods on the viability of pollens collected from Camellia azalea were investigated，using the MTT staining method,TTC staining method and pollen germination in vitro method. The results showed that the viability of pollens was associated with the testing method,the pollen germination in vitro was the optimum method to determine pollen viability of C.azalea. The pollen germinating rate reached the highest on the media supplemented with 2.5% agar，100 g/L sucrose and 10 mg/L boric acid under 30℃ after 4 hours.However，adding the Ca2+ on the media would prevent the pollen germinating.92.5% pollen had viability in MTT solution，significantly higher than by pollen germination in vitro.TTC staining method was unsuited for detection the pollen viability of C.azalea，for its dying unclearly and lower viability rate.

References:

[1]高继银,Clifford R P,杜跃强.山茶属植物主要原种彩色图集[M].杭州:浙江科学技术出版社,2005:34-35.[2]]李天菲，林田，徐碧玉，等.杜鹃红山茶花粉萌发力及贮藏耐性的研究[J].生物技术通报,2008(增刊):239-243.[3]严丹峰,李建光,许宇山,等.杜鹃红山茶花粉活力与柱头可授性研究[J].北方园艺,2013(2):71-73.[4]刘玉玲,潘文,张方秋,等.杜鹃红山茶花粉保存及其生活力测定[J].广东林业科技,2010,26(2):1-6.[5]赵鸿杰,乔龙巴图,殷爱华,等.3种山属属植物花粉活力测定方法的比较[J].中南林业科技大学学报,2010,30(3):105-107.[6]左丹丹,明军,刘春,等.植物花粉生活力检测技术进展[J].安徽农业科学,2007,35(16):4742-4745.[7]张超仪,耿兴敏.六种杜鹃花属植物花粉活力测定方法的比较研究[J].植物科学学报,2012,30(1):92-99.[8]罗晓莹.杜鹃红山茶保护生物学研究[D].广州:华南农业大学,2006.[9]汤红明,徐冬青,徐根娣,等.植物花粉萌发的研究进展[J].安徽农业科学,2006,34(24):6436-6438.[10]朱展望,张改生,牛娜.小麦花粉的离体萌发研究[J].麦类作物学报,2007,27(1):12-15.[11]白天,解玮佳,李世峰,等.糖、硼、钙对大喇叭杜鹃四合花粉萌发的影响[J].上海交通大学学报(农业科学版),2013,31(6):48-54.[12]刘赢男,周丹,刘伟,等.不同培养基及贮藏条件对山梅花属植物花粉生活力的影响[J].东北林业大学学报,2011,39(12):47-49.[13]车代弟,樊金萍,王金刚.东方百合花粉萌发培养基组分的优化[J].植物研究,2003,23(2):178-181.[14]Tian H Q,Zhang Z,Russell S D.Isolation of the male germ unit organization and function in tobacco(Nicotiana tabacum L.)[J].Plant Cell Reports,1998,18(12):143-147.[15]贾文庆,刘宇,陈韵,等.Ca2+与葱兰花粉萌发和花粉管生长的关系[J].西北林学院学报,2007,22(4):98-99.[16]Malho R，Read N D,Trewavas A J,et al.Calcium channel activity during pollen tube growth and reorientation[J].The Plant Cell,1995,7(8):1173-1184.[17]罗凤霞,周威,杨春起,等.水仙花粉离体萌发温度和培养液研究[J].辽宁林业科技,2007(5):13-16.[18]Graaf B H，Derksen J W，Marianic.Pollen and pistil in the programic phase[J].Sex Plant Report，2001,14:41-55.[19]周莉花,郝日明,赵宏波.蜡梅花粉活力检测方法筛选及保存时间观察[J].浙江林学院学报,2006,23(3):270-274.[20]杨秀莲，向其柏.桂花花粉活力测定与‘晚籽银’桂柱头可授性分析[J].林业科技开发,2007,21(3):22-25.

Memo

Memo:
-
Last Update: 2015-08-21