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Research on Rapid Propagation Technology of Two Lily Commercial Varieties(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年12
Page:
96-99
Research Field:
Publishing date:

Info

Title:
Research on Rapid Propagation Technology of Two Lily Commercial Varieties
Author(s):
WU Qing-qing1; DOU Yun2; ZHANG Chao-jun 1; SHI Le-juan 1; HU Xiao-jing 2
1.Horticulture Research Institute of Guizhou，Guiyang，Guizhou 550006；2.College of Horticulture，Guizhou University，Guiyang，Guizhou 550006
Keywords:
lily; explant; tissue culture
PACS:
S 682.2+65
DOI:
10.11937/bfyy.201512025
Abstract:
Taking the bulb scales of lily commercial oriental varieties of ‘Sorbonne’and‘Corvara’as the test materials and MS medium as the minimal medium，the experimental effect and screen the optimal formula with addition of plant growth regulator and sucrose of different concentrations was compared to lily tissne cultured seedling.The results showed that the optimal sterilizing time of explant was 10 min.The optimal formula for the inducing medium of adventitious buds of ‘Sorbonne’ was MS+sucrose 30 g/L+0.8% agar+6-BA 0.8 mg/L+NAA 0.1 g/L，the suitable proliferation medium was MS+sucrose 30 g/L+0.8% agar+6-BA 0.8 mg/L.The optimal formula for the inducing medium of adventitious buds of‘Corvara’was MS+sucrose 30 g/L+0.8% agar+6-BA 0.2 mg/L+NAA 0.1 g/L，the suitable proliferation medium was MS+sucrose 30 g/L+0.8% agar+6-BA 0.6 mg/L.High concentration sucrose was adopted as replacement of hormone for proliferation in the proliferation test of adventitious buds and the optimal formula of medium was MS+sucrose 60 g/L+0.8% agar.The optimal rooting medium was MS+sucrose 30 g/L+0.8% agar+NAA 0.1 g/L.

References:

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Last Update: 2015-08-09