|Table of Contents|

Analysis of Genetic Diversity and Construction of Fingerprint of Lycium bararum L.Using SSR Technology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年12
Page:
91-95
Research Field:
Publishing date:

Info

Title:
Analysis of Genetic Diversity and Construction of Fingerprint of Lycium bararum L.Using SSR Technology
Author(s):
SHAO Qian-shun 1; GAO Lei 1; NAN Xiong-xiong 1; XU Mei-long 2; LI Yong-hua 1; WANG Jin-xiu 1
1.Combined National and Provincial Center of Engineering and Research for Cultivation and Utilization of Northwestem Special Economical Forestry，Yinchuan,Ningxia 750004；2.Wolfberry Engineering and Technology Research Center of State Forestry Administration，Ningxia Forestry Institute，Yinchuan,Ningxia 750004
Keywords:
Lycium bararum L.; SSR; genetic diversity; fingerprint map
PACS:
S 567.1+9
DOI:
10.11937/bfyy.201512024
Abstract:
Genetic diversity of Ningxia wolfberry series varieties and other Chinese wolfberry species/varieties and wild type were analyzed using Simple Repeat sequences (SSR) technology with 17 wolfberry germplasm，also their fingerprint map was constructed for the first time.The results showed that 17 Chinese wolfberry germplasm was checked with 15 pairs of SSR primers，and 91 polymorphic loci of 109 loci were obtained，the proportion of polymorphism was 83.4%；the average diversity index H of 17 germplasm was 0.1943，the Shannon information index I was 0.3108；the Nei ‘ s diversity index H of 10 ningxia wolfberry cultivars was 0.0962，the Shannon information index I was 0.1537；the Nei ’s diversity index H of 7 wild germplasm was 0.2004，Shannon information index I was 0.3130.It’s proved that the diversity of the not cultivated wolfberry was richer than that of cultivated varieties.The fingerprint map of 17 Chinese wolfberry germplasm was constructed with four pairs of primers.Our study laid a solid foundation for the identification of Chinese wolfberry species and the construction of genetic map.

References:

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Last Update: 2015-08-09