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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2015年12

Page:

76-79

Research Field:

Publishing date:

Info

Title:

SSR Loci Information Analysis in Nervilia fordii Transcriptome

Author(s):

HUANG Qiong-lin ¹; HE Rui ²; ZHAN Ruo-ting ²; CHEN Wei-wen ²

1.Guangdong Medical University，Zhanjiang,Guangdong 524023;2.Research Center of Chinese Medicinal Resource Science and Engineering，Guangzhou University of Chinese Medicine；Key Laboratory of Chinese Medicinal Resource from Lingnan，Ministry of Education，Guangzhou,Guangdong 510006

Keywords:

Nervilia fordii; SSR; loci information; transcriptome

PACS:

R 282.71

DOI:

10.11937/bfyy.201512021

Abstract:

Taking 142 220 unigenes in Nervilia fordii transcriptome as materials to identify functional simple sequence repeat (SSR)，which would promote the researches on resource protection including genetic diversity，molecular breeding and genetic mapping of N.fordii. The distribution frequency of high flux transcriptome SSR and the basic characteristics of repeat motifs were analyzed using MicroSAtellite software，and then SSR primers were designed by Primer 5.0 software and validated by SSR Finder software The results showed that 5 684 SSR loci were acquired from 5 223 unigenes with an occurrence frequency of 3.67% and a density of 1/13 kb.Dinucleotide repeat was the main SSR type of N. fordii，and AG/CT and AT/AT exhibited the highest occurrence frequency (52.9%) among all SSR motifs.A total of 2 486 primer pairs were designed for marker development.

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Last Update: 2015-08-09