|Table of Contents|

Optimizating of Aerial Root Chromosome Preparation Technology and Karyotype Analysis of Red Pitaya( Hylocereus polyrhizus)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年12
Page:
71-76
Research Field:
Publishing date:

Info

Title:
Optimizating of Aerial Root Chromosome Preparation Technology and Karyotype Analysis of Red Pitaya( Hylocereus polyrhizus)
Author(s):
LIU Shun-zhi; ZHOU Hao-bin; LIN Run-yi; QIN Xi; WANG Yu-lin; HU Wei-rong
School of Life Sciences，Guangzhou University，Guangzhou，Guangdong 510006
Keywords:
red pitaya; aerial root; chromosome; preparation technique; karyotype analysis
PACS:
S 667.9
DOI:
10.11937/bfyy.201512020
Abstract:
Taking aerial root tips of red pitaya (Hylocereus polyrhizus) as material for chromosome preparation，the effect of root length，sampling time，permanent，dissociation time and staining on cytological observation were analyzed. The results showed that the optimum chromosome preparation was achieved with collecting root tips at 10:30 AM，root length within 5 mm，pretreated for 2 hours in 0.1% colchicine at room temperature，spreading chromosomes by double hypotonic treatment，dissociation with 1 mol/L HCl for 50 minutes at room temperature or 20 minutes at 60℃，and stained with 20% modified carbol fuchsin dyeing.The permanent slides could be made by the neutral resin.The karyotype analysis indicated that red pitaya is diploid(2n＝2x＝22)，and the ratio of the longest chromosome to the shortest one was 1.63，so that the karyotype formula of red pitaya was 2n＝22m，belonging to 1A karyotype.The karyotype asymmetrical coefficient was 54.29%.

References:

[1]邓仁菊，范建新，蔡永强.国内外火龙果研究进展及产业发展现状[J].贵州农业科学,2011,39(6):188-192.[2]Herbach K M，Rohe M，Stintzing F C，et al.Structural and chromatic stability of purple pitaya (Hylocereus polyrhizus［Weber］ Britton & Rose) betacyanins as affected by the juice matrix and selected additives[J].Food Research International,2006,39(6):667-677.[3] Nerd A,Sitrit Y,Kaushik R A,et al.High summer temperatures inhibit flowering in vine pitaya crops (Hylocereus spp.)[J].Scientia Horticulturae,2002,96:343-350.[4]Tao J，Qiao G，Wen X P，et al.Characterization of genetic relationship of dragon fruit accessions(Hylocereus spp.) by morphological traits and ISSR markers[J].Sci Hortic,2014,170:82-88.[5]陈丽娜,陈石,李润唐,等.红肉火龙果与白肉火龙果品质分析[J].中国南方果树,2011,40(4):69-70.[6]刘顺枝,孙茹,江月玲,等.贮藏温度对火龙果品质和衰老变化的影响[J].食品科学,2013,34(12):336-340.[7]Guo L W，Wu Y X，Ho H H，et al.First report of dragon fruit (Hylocereus undatus) anthracnose caused by Colletotrichum truncatum in China [J]. Journal of Phytopathology,2014,162(4):272-275.[8] Cisneros R，Garcia R B，Tel Zur N.Ovule morphology,embryogenesis and seed development in three Hylocereus species(Cactaceae)[J].Flora,2011,206:1076-1084.[9]Tel Zur N,Dudai M,Raveh E,et al.In situ induction of chromosome doubling in vine cacti(Cactaceae)[J].Scientia Horticulturae,2011,129:570-576. [10]杨汉民.细胞生物学实验[M].2版.北京:高等教育出版社,1997. [11]张红梅,张蜀宁,孔艳娥,等.青花菜染色体制片技术及核型分析[J].南京农业大学学报,2009,32(4):33-36.[12]何兰花,卢家现.用改良苯酚品红染色液替代醋酸洋红染色液的研究[J].生物学杂志,2000,17(2):28-29.[13]Chen R Y,Song W Q，Li X L.A new method for preparing mitotic chromosomes from plant[J].Acta Bot Sin,1979,21(3):297-298.[14]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302.[15]Stebbins G L.Chromosome evolution in higher plants[M].London:Edward Arnold,1971;87-89.[16]刘敏,王卉,宁慧霞,等.天山雪莲根尖染色体制片影响因素研究及组型分析[J].西北农业学报,2013,22(2);170-176.[17]Tel Zur N，Abbo S，Bar Zvi D，et al.Genetic relationships among Hylocereus and Selenicereus vine cacti (Cactaceae)：evidence from hybridization and cytological studies[J].Ann Bot,2004,94(4):527-534.[18]Arano H.Cytological studies in subfamily earduoideae （compositae) of Japan.IX [J].Bot Mag,1963,76:32-39.[19]刘顺枝,刘正浩,林润怡,等.白肉火龙果染色体制片技术及核型分析[J].广东农业科学,2015,42(3):115-118.

Memo

Memo:
-
Last Update: 2015-08-09