Establishment of Regeneration System in the Rachis of Garlic(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: LIU Wei-wei; SONG Shu-min; PAN Jing; GAO Yu; (Daqing Branch，Heilongjiang Academy of Siences，Daqing,Heilongjiang 163319)

Abstract:: Taking rachis garlic as test material，using plant tissue culture method，the effect of different concentrations of cytokinin，sucrose concentration and culture medium type on rachis of garlic were studied.The results showed that induction differentiation of rachis was the best when the medium was MS containing 6-BA 1.5 mg/L，NAA 0.1 mg/L and sucrose 30 g/L at pH 5.8,the differentiation rate was 100%,the proliferation ratio was 46.3.The suitable subculture conditions were 1/2 MS medium containing 6-BA 0.1 mg/L，NAA 0.5 mg/L，subsisr rate was 100%,and grew strongly.

[1]梁艳,陈典,黄晓梅,等.大蒜试管微鳞茎形成的激素筛选研究[J].东北农业大学学报,2005,36(5):589.

[2]Ma Y，Wang H L，Zhang C J,et al.High rate of virus-free plantlet regeneration via garlic scape-tip culture[J].Plant Cell Reports,1994，14(1)：65-68.

[3]陈典.大蒜脱毒种苗培育技术研究[J].北方园艺,1997(2):29-30.

[4]李昌华,李小川,赵美华,等.大蒜茎尖脱毒技术及组织培养研究[J].华北农学报,1995,10(3):20-25.

[5]熊正琴,李式军,刘高琼,等.大蒜花序轴离体培养的研究[J].南京农业大学学报,2000,23(3):25-28.

[6]周桂珍,曹鸣庆,裘季燕,等.京郊大蒜病毒的研究及其鳞茎中病毒的脱除[J].植物病理学报,1989,19(3):145-149.

[7]林碧英,傅睿清.植物激素配比对大蒜鳞茎盘培养体系的影响[J].福建农业科技,2008(6):71-72.

[8]栾非时,陈典,陈友.脱毒大蒜花原始体培养增殖技术的研究[J].中国蔬菜,1995(3):4-6.

Last Update: 2015-08-08