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The Activity of Key Enzymes Related to Flavonoids in Test-tube Plantlets of Hippophae neurocarpa Under Low Temperature(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年10
Page:
5-8
Research Field:
Publishing date:

Info

Title:
The Activity of Key Enzymes Related to Flavonoids in Test-tube Plantlets of Hippophae neurocarpa Under Low Temperature
Author(s):
ZHANG Hong-taoCHEN WenLI Xiao-weiSU XueSUN Kun
(College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070)
Keywords:
Hippophae neurocarpatest-tube plantletslow-temperature stressPALC4H4CLenzyme activity
PACS:
S 793.6
DOI:
10.11937/bfyy.201510002
Abstract:
Test-tube plantlets leaves of Hippophae neurocarpa was used as materials,activities of three key enzymes related to flavonoids,phenylalanine ammonialyase (PAL),4-hydroxy cinnamic acid enzyme (C4H) and 4-Coumadin acid coenzyme A ligase (4CL) were tested under low temperature in test-tube plantlets leaves of Hippophae neurocarpa.The results showed that the three enzymes could be affected easily under low temperature.In the early stage of stress,activities of PAL and C4H fluctuated greatly.The activity of PAL reached the highest value at 12 hours and got the lowest value by 24 hours,which was 63.514% lower than that of control.Its activity recovered gradually afterwards.The activity of C4H got the lowest value at 12 hours and began to increase after that.Its activity was 447.2% of the control by 72 hours.The activity of 4CL reached the highest after 6 hours and was 36.285% higher than that of control.The activity of 4CL decreased after that and was lower than that of control at last.The activities of PAL,C4H and 4CL in test-tube plantlets leaves of Hippophae neurocarpa changed in different ways under low temperature.Phenylaprapanoid metabolism was activated and synthesis of secondary metabolites such as flavonoids could be regulated.

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Last Update: 2015-08-05