Selection and Identification of Hybrid Strain of Pleurotus eryngii var. tuoliensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Selection and Identification of Hybrid Strain of Pleurotus eryngii var. tuoliensis

Author(s):: GAO Kun-mei; XING Zhi-wei; ZHENG Su-yue; LU Yue-xia; (College of Agriculture，Hebei University of Engineering，Handan,Hebei 056038)

Keywords:: Pleurotus eryngii var. tuoliensis; hybrid strain; antagonistic test; isozyme analysis; primordium formation

Abstract:: Taking two different Pleurotus eryngii var. tuoliensis strains as parents (‘Zhongnong No.1’ and‘Zhongnong Chibao’),the proterties of 150 cross combinations were studied by microscopy，antagonism，esterase isozyme and primordium induction.The results showed that 99 of 150 hybrid strains were with clamp-connection，75 strains had obvious antagonism with two parents and had new enzyme bands and complementary enzyme bands，50 strains had primordium formation in PDA plate.

[1]王贺祥，刘庆洪.食用菌栽培学[M].北京：中国农业大学出版社，2014.

[2]桂明英，王刚，郭永红，等.食用菌育种技术的研究进展[J].中国食用菌，2006，25(5)：3-5.

[3]郑素月，卢月霞，张庆桥.1种简单的白灵菇单孢分离新方法[J].中国食用菌，2013，32(5)：14-15.

[4]胡能书，万贤国.同工酶技术及其应用[M].长沙：湖南科学技术出版社，1985.

[5]马银鹏.环境因子对白灵侧耳原基形成的影响研究[D].北京:中国农业科学院，2012.

[6]刘宇，陈文良，王丽珍，等.杏鲍菇13 号杂交菌株选育研究[J].食用菌学报，2004，11(3)：61-64.

[7]刘宇，陈文良，耿小丽，等.杏鲍菇16号杂交菌株选育[J].食用菌，2009(2)：23-24.

Last Update: 2015-08-02