|Table of Contents|

Molecular Detection and Identification of Tomato chlorosis virus Infecting Greenhouse-grown Tomato Plants in Hebei Province(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年09
Page:
95-98
Research Field:
Publishing date:

Info

Title:
Molecular Detection and Identification of Tomato chlorosis virus Infecting Greenhouse-grown Tomato Plants in Hebei Province
Author(s):
SUN Guo-zhen1; GAO Li-li1; LU Wen-li2; WANG Yong3; ZHANG An-sheng4; ZHU Xiao-ping1
(1.College of Plant Protection,Shandong Agricultural University,Tai’an，Shandong 271018;2.College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018;3.Tianjin Institute of Plant Protection,Tianjin 300381;4.Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan，Shandong 251000)
Keywords:
Tomato chlorosis virus; HSP70; CP; RT-PCR detection; Tomato yellow leaf curl virus; mixed infection
PACS:
S 641.2
DOI:
10.11937/bfyy.201509026
Abstract:
Taking tomato samples showing symptom similar to that caused by Tomato chlorosis virus (ToCV) found and collected from 7 regions in Hebei Province in the summer of 2014 as material，using RT-PCR detection，BLAST alignment and phylogenetic analysis based on MEGA 5.0 as research methods to detect and identify ToCV occurrence and mixed infection on ToCV and another economic important virus Tomato yellow leaf curl virus (TYLCV).The results showed that three pairs of specific primers targeting the ToCV coat protein (CP) and heat shock protein (HSP70) genes were used for detection，specific 1 032 bp，943 bp and 911 bp fragments were amplified from the symptomatic samples，respectively.The ToCV positive samples were also tested for presence of TYLCV，specific primers of TYLCV were used and a 833 bp specific fragments were amplified and sequenced.Plant samples from Hebei were infected by Tomato chlorosis virus，the sequence analyses showed the nucleotide sequence of Hebei samples had the highest identity of 99.7% with a Beijing isolate of ToCV (KC887999).The mixed infection of ToCV and TYLCV occurred in the field was also confirmed.

References:


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Last Update: 2015-08-01