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Screening and Expression of CCoAOMT and LAR Genes from Nai’s Fruit(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年09
Page:
87-94
Research Field:
Publishing date:

Info

Title:
Screening and Expression of CCoAOMT and LAR Genes from Nai’s Fruit
Author(s):
CHEN Gui-xin1; 2 ; WANG Yu-zhen1; 2 ; ZHAO Li1; 2 ; JIANG Cui-cui3; LYU Shi-heng1; PAN Dong-ming1; 2
（1.College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou,Fujian 350002;2.Institute of Storage Science and Technology of Horticultural Products，Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;3.Fruit Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou,Fujian 350013）
Keywords:
Nai（Prunus salicina）; PsCCoAOMT; PsLAR; dilute-pool PCR; real-time PCR
PACS:
S 665.9
DOI:
10.11937/bfyy.201509025
Abstract:
Dynamic changes of expression of PsCCoAOMT and PsLAR genes at different development stages in Nai’s fruits were explored，a normalized full-length cDNA library was constructed with Nai’s fruits at different developmental stages as research materials by combination of two-step reverse transcription and suppressive PCR.Full-length cDNAs of PsCCoAOMT and PsLAR encoding key enzymes in metabolic pathway of phenolics in Nai’s fruit were screened by dilute- poold PCR method.The results showed that the full length cDNAs of PsCCoAOMT and PsLAR genes were 1 195 bp with 744 bp of ORF encoding 247 amino acids and 1 625 bp with 1 050 bp of ORF encodingand 349 amino acids,respectively.The protein encoded by PsCCoAOMT was a stable hydrophilic protein whose relative molecular weight ofmolecular weight was 27 893.8 and pI was 5.42；the protein encoded by PsLAR was an unstable hydrophilic protein whose relative molecular weight ofmolecular weight was 38 483.1 and pI was 5.40.Homologous analysis showed that the homology of PsCCoAOMT compared with that of Pyrus x bretschneideri was the highest by 96%，the PsLARs were highly homologous in Rosaceous plants.The results of Real-time PCR showed that the change of PsCCoAOMT was not obvious and tended to reduce overally in the whole process of development of fruit and reached to minimum after 50 days of anthesis；that of PsLAR gradually reduced in the whole process of development of fruit，that of the gene reduced to zero after 125 days of anthesis.

References:


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Last Update: 2015-08-01