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The Establishment of Asparagus officinalis Tissue Culture and Rapid Propagation System(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年09
Page:
83-86
Research Field:
Publishing date:

Info

Title:
The Establishment of Asparagus officinalis Tissue Culture and Rapid Propagation System
Author(s):
LU Zhi-wei1; GAO Jian-ming1; 2 ; LIU Qiao-lian2; HOU Xiao-wan2; YI Ke-xian2
(1.College of Agronomy，Hainan University，Haikou，Hainan 570228；2.Institute of Environment and Plant Protection Institute，CATAS，Haikou，Hainan 571101)
Keywords:
Asparagus officinalis; embryos; tissue culture; rapid propagation
PACS:
S 644.603.6
DOI:
10.11937/bfyy.201509024
Abstract:
The sterile shoot tips of high-yielding varieties of ‘Jinggang 701’ Asparagus officinals were used as experimental materials，using orthogonal design,the effect of different concentrations combinations of pyrimidinol，KT，2,4-D ，abscisic acid，6-BA and NAA on the inductions for callus，somatic embryoids and seedlings were studied.The results showed that the optimum inducing medium for callus was MS+2,4-D 2 mg/L+KT 1.0 mg/L,the inducing rate reached to 80％；the optimum inducing medium for embryos was MS+0.70 mg/L ancymidol+0.10 mg/L NAA+0.50 mg/L KT，the inducing rate reached to 95％；the optimum inducing medium for plantlet was MS+NAA 0.5 mg/L+6-BA 1.0 mg/L ，the inducing rate reached to 60％；the survival rate of outdoor transplant could be about 90％.The system of one-step inducing into plantlet for asparagus was initially established.

References:


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Last Update: 2015-08-01