|Table of Contents|

Study on Regeneration of Adventitious Bud from Leaves of Pyrus sinkiangensis Yü(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年09
Page:
76-79
Research Field:
Publishing date:

Info

Title:
Study on Regeneration of Adventitious Bud from Leaves of Pyrus sinkiangensis Yü
Author(s):
QIN Lu; CHEN Quan; LIANG Zhi-qiang; ZHU Jian-bo
(College of Life Science,Shihezi University,Shihezi,Xinjiang 832000)〖HQ〗
Keywords:
Korlas frant pear (Pyrus sinkiangensis Yü); regeneration from leaves; adventitious buds
PACS:
S 661.203.6
DOI:
10.11937/bfyy.201509022
Abstract:
Taking Korlas frant pear (Pyru ssinkiangensis Yü) young expanded leaves extracted as explants，the effect of element composition of basic medium，the concentration of plant growth regulators，AgNO3 concentration and leaf direction on the medium on shoot regeneration were studied.The results showed that the element composition of basic media was the major factor affecting the shoot regeneration.NN69 medium was the optimal basic medium for the adventitious shoot regeneration from leaves of the Pyru ssinkiangensis Yü and the optimum medium was NN69 medium with 1.0 mg/L thidiazuron and 0.3 mg/L indole-3-butytric acid.AgNO3 enhanced the regeneration efficiency while its concentration was 0.5 mg/L.Adventitious buds was easier induced with the leaf abaxial side touching the medium than with the other side.After 21 days dark culture，the maximum regeneration frequency was 64.3% and the maximum bud number per explant was 2.59.

References:


[1]王国平,洪霓,张尊平,等.我国北方梨产区主栽品种病毒种类的鉴定研究[J].中国果树,1994(2):1-4.
[2]洪霓,王国平,张尊平,等.梨病毒脱除技术研究[J].中国果树,1995(4):5-7,24.
[3]Lane D.Regeneration of Pear plants from shoot meristem tips[J].Plant Science Lett,1979(13):181-285.
[4]赵惠祥.梨茎尖离体培养[J].Journal of Integrative Plant Biology,1982(4):392-394.
[5]Laimer M,Camara M A,Hanzer V,et al.Regeneration of shoots from leaf discs and stem microcuttings of fruit trees as a tool for transformation[J].Acta Horticulturase,1988,235:85-92.
[6]薛光荣,杨振英,史永忠,等.锦丰梨花粉植株的诱导[J].园艺学报,1996(2):123-127.
[7]薛光荣,杨振英,洪霓,等.茎尖培养等处理脱除梨病毒的技术研究[J].中国果树,1996(3):9-11.
[8]孙清荣.金花梨叶片不定梢诱导研究[J].落叶果树,2000(3):8-10.
[9]孙清荣,孙洪雁,刘庆忠.丰水梨的组培快繁研究[J].落叶果树,2001(4):4-5.
[10]孙清荣.影响苹果叶片不定芽再生的几个因素[J].落叶果树,1995(4):3-4.
[11]Lane W D,Iketani H,Hayashi T.Shoot regeneration form cultured leaves of Japanese(Pyrus pyrifolia)[J].Plant Cell Tissue Organ Culture,1998,54(1):9-14.
[12]王莉萍.阿月浑子和库尔勒香梨微繁殖技术研究[D].乌鲁木齐：新疆农业大学,2004.
[13]张志宏,景士西,王关林.TDZ对苹果叶片离体再生不定芽的效应(简报)[J].植物生理学通讯,1997(6):420-423.
[14]孙清荣,樊圣华,刘庆忠.大鸭梨叶片高频率不定梢再生诱导研究[J].山东农业科学,2003(2):10-12.
[15]Caboni E,Tonelli M G,Lauri P,et al.In vitro shoot regeneration from leaves of wild pear[J].Plant Cell,Tissue and Organ Culture,1999,59(1):1-7 .
[16]路娟,吴俊,张绍铃,等.不同系统梨种质遗传多样性与分类关系的SSR分析[J].南京农业大学学报,2011(2):38-46.
[17]陶爱群,尹婷,谢深喜.黄金梨叶片再生体系建立研究[J].湖南农业科学,2012(5):108-111.

Memo

Memo:
-
Last Update: 2015-08-01