|Table of Contents|

Screening of Lentinula edodes with High Exopolysaccharide inQinling-Bashan Mountainous Area(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年08
Page:
137-142
Research Field:
Publishing date:

Info

Title:
Screening of Lentinula edodes with High Exopolysaccharide inQinling-Bashan Mountainous Area
Author(s):
QIAO Yan-ming1; CHEN Wen-qiang1; 2 ; DENG Bai-wan1; 2 ; CHENG Xian-li3 ; PENG Hao1; 2 ; WANG Yu1
(1.School of Biological Science and Engineering，Shaanxi University of Technology，Hanzhong，Shannxi 723001；2.Shaanxi Engineering Research Center of Edible and Medicated Fungi，Hanzhong，Shannxi 723001；3.Department of Pharmaceutical and Medical Technology，Hanzhong Vocational and Technical College，Hanzhong，Shannxi 723000)
Keywords:
Lentinula edodes; exopolysaccharide; screening; liquid fermentation
PACS:
S 646.1+2
DOI:
10.11937/bfyy.201508036
Abstract:
Using tissue culture method，with Qinba Mountain main cultivated Lentinula edodes (808#，66#，68#) as test materials，Lentinula edodes with high exopolysaccharides was selected by 6 kinds of screening medium.The mycelial growth rate of Lentinula edodes strains and the exopolysaccharides production of Lentinula edodes with high exopolysaccharides were studied.The results showed that the hypha of Lentinula edodes (66#) was thick and dense and hypha growth rate was 3.26 mm/d in screening medium (glucose 1.0%，corn flour 2.0%，wheat bran 2.0%，yeast extract 0.3%，KH2PO4 0.1%，MgSO4? 7H2O 0.1%，pH naturally).There were many mycelia spheres to be evolved and the size of mycelia spheres was even，and the mycelia amount of biological transformation (wet weight) was up to 66.00 g/L in screening medium.The exopolysaccharides production of Lentinula edodes (66#) was up to 2.99 g/L.

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Last Update: 2015-08-07