Callus Induction and Establishment of the Regeneration System of Peperomia obtusifolia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2015年08

Page:

117-120

Research Field:

Publishing date:

Info

Title:

Callus Induction and Establishment of the Regeneration System of Peperomia obtusifolia

Author(s):

GUO Chen-chen1; LEI YA-zhen1; LIU Chun2; ZHANG Li1

(1.School of Agriculture，Ningxia University，Yinchuan，Ningxia 750021;2.Research Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081)

Keywords:

Peperomia obtusifolia;  explant; regeneration system; calus; induction

PACS:

S 682.36

DOI:

10.11937/bfyy.201508031

Abstract:

The leaves，petioles and stems with buds of Peperomia obtusifolia were chosen as the explants to select the best explant types，the combinations of 6-BA and IBA was used to select suitable medium of callus induction and bud multiplication；activated carbon was used to screen the appropriate rooting medium.The results showed that the stems with buds was suitable for inducing callus and the browning rate was only 6.7%，and the leaf and petiole browning rate were more than 60%.It indicated that the optimum medium for callus induction was MS+6-BA 2.0 mg/L+IBA 0.1 mg/L and the rate of callus was 100.00%;the best shoot proliferation was MS+6-BA 0.5 mg/L+IBA 0.1 mg/L and the multiplication coefficient was 13.8；the optimum root rooting medium was 1/2 MS+IBA 0.2 mg/L+AC 1.0 g/L.

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