Study on the Method of Measuring Pollen Viability and Pretreatment of Pollen(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Method of Measuring Pollen Viability and Pretreatment of Pollen

Author(s):: YANG Hong¹; YU He-ming²; LI Xiao-yan¹; FENG Ying-ying¹; （1.College of Animal Science，Xichang College，Xichang，Sichuan 615013；2.Tobacco Scientific Research Institute，Tobacco Company of Liangshan，Xichang，Sichuan 615013）

Keywords:: Actinidia chinensis; pollen viability; pollen germination; staining; germination in vitro

Abstract:: Method of measuring pollen viability and pretreatment of pollen have very important significance for artificial pollination.Taking Actinidia chinensis as materials，the best way of measuring pollen viability and the effect of different treatments were studied.The results showed that,for pollen of flower bud，the method of TTC，the pollen viability was low，and the method of germination in vitro and the pollen viability was high.So，the best way of measuring pollen viability was methods of staining and germination.The rate of pollen germination was associated with the time，and for flower bud，the best way was separation of stamens and petals immediately for improving the rate of pollen germination；Pollen drying was associated with environmental condition，if the environment was suitable for drying pollen，it didn’t need to use other drying methods.

[1]李文生,石磊,王宝刚,等.不同颜色果肉猕猴桃营养品质的比较[J].食品科技,2012,37(7):47-52.

[2]艾应伟,范志金.四川盆周山区发展特色优质猕猴桃的优势与对策[J].园艺园林科学，2005,21(11):259-261.

[3]陈学森,李杨,束怀瑞.果树开花授粉生物学研究进展[J].山东农业大学学报(自然科学版),2000,31(3):345-348.

[4]陈永安,陈鑫,刘艳飞.采粉期及贮藏条件对猕猴桃花粉生活力的影响[J].西北农林科技大学学报(自然科学版),2012,40(8):157-160.

[5]陈延惠,李洪涛,朱道圩,等.猕猴桃花粉生活力及其贮藏性的研究[J].河南农业大学学报，1996,30(2):175-177.

[6]姚春潮,龙周侠,刘旭锋,等.不同干燥及贮藏方法对猕猴桃花粉活力的影响[J].北方园艺,2010(20):37-39.

[7]姚春潮,张朝红,刘旭锋,等.猕猴桃花粉萌发动态及培养基成分对花粉萌发的影响[J].中国南方果树,2005,34(2):50-51.

[8]李平,张朝红,艾绍周,等.猕猴桃花粉萌发的影响因子[J].北方果树,2007(1):5-7.

[9]齐秀娟,张绍铃,方金豹.培养环境条件对猕猴桃花粉萌发的影响[J].浙江农业学报,2011，23(3):528-532.

Last Update: 2015-07-22