Optimization of Ultrasound-assisted Extraction of Manyprickle acathopanax Root Polysaccharides Using Response Surface Methodology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Ultrasound-assisted Extraction of Manyprickle acathopanax Root Polysaccharides Using Response Surface Methodology

Author(s):: CHEN Jin-e1; WANG Qing-hong1; WEI Zeng-yun2; ZHANG Hai-rong1; (1.Institute of Biochemical Analysis，Xinzhou Teachers’University，Xinzhou，Shanxi 034000；2.College of Xinzhou Occupation Technology，Xinzhou，Shanxi 034000)

Keywords:: response surface analysis; Manyprickle acathopanax; ultrasound-assisted extraction

Abstract:: Taking Manyprickle acathopanax as test material，the polysaccharides was extracted by ultrasound-assisted extraction technology.The quadratic polynomial regression equation prediction model was obtained by using the Box-behnken central composite test and response surface analysis based on the single factor experiments，such as ultrasound power (A)，temperature (B)，liquid-solid ratio (C)，and the ultrasound (D).The results showed that the optimal conditions of ultrasound-assisted extraction of Manyprickle acathopanax polysaccharides were as follows,ultrasound power of 90 W，extraction temperature of 57℃，40∶1 mL/g of the ratio of liquid to solid and time of 42 minutes.Under these conditions，the experimental yield of Manyprickle acathopanax polysaccharides was 3.86%，which was well matched with the value predicted (3.88%) by the model.Ultrasonic extraction method was not only low energy consumption，high yield，and the extraction time was shortened by 11 times compared with the traditional water-boiling method.

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Last Update: 2015-07-24