|Table of Contents|

Extraction Technology of Total Saponins from Alfalfa by Enzymatic Hydrolysis Method Assisted by Ultrasonic Technology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年07
Page:
116-118
Research Field:
Publishing date:

Info

Title:
Extraction Technology of Total Saponins from Alfalfa by Enzymatic Hydrolysis Method Assisted by Ultrasonic Technology
Author(s):
LI Xiao-an; YIN Wei; YANG Guo-zhu; TIAN Hai-ning; ZHANG Min; ZHANG Cheng-sheng
（College of Eco-Environmental Engineering，Qinghai University，Xining，Qinghai 810016）
Keywords:
Medicago sativa L.; total saponins; enzymatic hydrolysis method assisted by ultrasonic technology; orthogonal method
PACS:
-
DOI:
10.11937/bfyy.201507034
Abstract:
Taking new strain of alfalfa ‘Qingda No.1’ hay as raw material，the total saponins were extracted by enzymatic hydrolysis method assisted by ultrasonic technology，then total saponins extracted liquid with petroleum ether and n-butanol to two extraction，while determine the content of total saponins in Uv spectrophotometry.The ratio of solid to liquid（A），ultrasonic time（B）and ultrasonic temperature（C），ultrasonic power（D）as a single factor gradient test，based on single factor test results，using four factors (solid-liquid ratio，ultrasonic time，ultrasonic temperature，ultrasonic power) three levels orthogonal experiment design to optimize the extraction process,to develop new resources of alfalfa saponis.The results showed that the best ultrasonic method to extract the total saponins conditions was A3B1C1D2，the ratio of solid-liquid was 1∶30 g/mL，ultrasonic time was 20 miniutes，ultrasonic temperature was 20℃，ultrasonic power was 360 W.

References:

 

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Last Update: 2015-07-17