|Table of Contents|

Establishment of High Frequency Regeneration System of Portulace oleracea L.and Determination of Phosphinothricin Sensitivity(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年07
Page:
98-103
Research Field:
Publishing date:

Info

Title:
Establishment of High Frequency Regeneration System of Portulace oleracea L.and Determination of Phosphinothricin Sensitivity
Author(s):
LIU Bu-cang; ZHANG Ai-xia; LI Yu-shun; ZHU Jian-bo
(College of Life Science，Shihezi University,Shihezi，Xinjiang 832003)
Keywords:
Portulaca oleracea L.; high frequency plant regeneration system; phosphinothricin
PACS:
-
DOI:
10.11937/bfyy.201507029
Abstract:
Taking Portulaca oleracea L.cotyledon and hypocotyl as explants，the effect of exogenous hormone on the inducement of adventitious buds，and different concentration of 6-BA，NAA，GA3 on the elongation of adventitious buds were studied,the sensitivity of phosphinothricin of Portulaca oleracea L.cotyledon and hypocotyl were determined.The results showed that the optimum induction mediun of hypocotyl adventitious buds was MS+2.0 mg/L 6-BA+100 mg/L CH (casein acid hydrolysate）and the average induction rate was 96.65%；the optimum mediun of cotyledon adventitious buds induction was MS+3.0 mg/L 6-BA+100 mg/L CH(casein acid hydrolysate）and the average induction rate was 100%;the optimum shoot growth medium was MS+0.5 mg/L 6-BA+0.05 mg/L NAA+0.2 mg/L GA3；the rooting medium was MS+2.0 mg/L IBA with rooting rate 100%.Determination of the phosphinothricin sensitivity showed that compared to hypocotyl，cotyledon was more sensitive to phosphinothricin.At 0.050 mg/L PPT，the fatality rate was 89.0% and 71.5% in cotyledon and hypocotyl respectively.This study established the high frequency regeneration system of Portulace oleracea L.and determined its sensitivity to phosphinothricin.Results from this study would help the research of Portulaca oleracea L. in genetic engineering.

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Last Update: 2015-07-17