Study on Tissue Culture of Rhododendron fortunei in Xinning(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: TANG Ying-hong1; 2; SHEN Fan1; LIU Fang1; GUO Qing-quan1; KANG Yong-quan3; CHEN Jian-rong1; (1.Department of Biological and Environmental Engineering,Changsha University，Changsha,Hunan 410022；2.Agricultural College，Hunan Agricultural University，Changsha,Hunan 410128；3.Hunan Xiang Zhi Garden Technology Co.Ltd.，Changsha,Hunan 410116)

Abstract:: Taking leaves and stems of Rhododendron fortunei as explants，using plant tissue culture techniques，different explant disinfection programs were conducted and using 1/4MS as the basic medium supplemented with different concentrations of ZT and NAA plant growth regulating substances，in order to study the effect of different culture conditions on Rhododendron in vitro bud induction and proliferation.The results showed that the best explant disinfection program was 75% ethanol for 30 seconds and 5% sodium hypochlorite for 10 minutes，and the shoot apex had the highest induced rate.The best bud induction medium program was 1/4MS mixed with 7 mg/L ZT and 0.15 mg/L NAA，in vitro shoots had the best proliferation effect in medium with 1/4MS mixed with 1.0 mg/L ZT and 0.10 mg/L NAA,seedling had the best rooting effect in medium with 1/2MS+0.01 mg/L 6-BA+1.5 mg/L NAA.

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Last Update: 2015-07-17