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Effect of ABA,H2O2 and NO Donor SNP on the Gene Expression and Development of Jujube Fruit(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年07
Page:
90-93
Research Field:
Publishing date:

Info

Title:
Effect of ABA,H2O2 and NO Donor SNP on the Gene Expression and Development of Jujube Fruit
Author(s):
YANG Wei-min; DU Jing-qi; ZHAO Jun; CHU Pan-pan; LIU Bao-qi
（Department of Life Science，Lyuliang University,Lyuliang，Shanxi 033001）
Keywords:
Mu jujube; ABA; H2O2; NO donor SNP; DNA; gene expression
PACS:
-
DOI:
10.11937/bfyy.201507027
Abstract:
The jujube encounter overcast and rainy weather occurs the phenomenon serious in the mature period,the physiological disease gene expression associated with abiotic or biotic stress based on.Taking Lyuliang local Mu jujube as test material,in the jujube mature period,detection for change of DNA purity and contentby of tissue by the modified CTAB method, gene expression of ABA,H2O2 and NO in fruit development were studied.The results showed that effect of ABA，H2O2 and NO donor sodium nitroprusside (SNP) on purity and conten of DNA in fruit development had significant difference.Treated with different concentrations of ABA in jujube fruit，the DNA OD260/OD280 ratio was not exactly in the range of 1.700-1.900，the purity of DNA was obvious difference.But the content of DNA was higher, its value at 1 200 ng/μL; the DNA OD260/OD280 ratio was basically in between 1.700-1.900 with H2O2 and SNP treated，DNA with high purity. The content of DNA in the sample basically or less than or at 1 000 ng/μL.The gene expression were obviously interfered with exogenous ABA,and induced biosynthesis of new proteins，RNA，and the phenolic compounds，had a tendency to promote the development of jujube fruit；While effect of H2O2 and NO on gene expression or in fruit development was not very obvious.

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Last Update: 2015-07-16