Analysis on Esterase Isozemes and Peroxidase Isozymes in Singlecell Clone of Scutellaria Callus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Analysis on Esterase Isozemes and Peroxidase Isozymes in Singlecell Clone of Scutellaria Callus

Author(s):: LIU Li-jie; LIN Li-dong; ZHANG Dong-xiang; (College of Life Science and Agriculture-Forestry，Qiqihar University，Qiqihar,Heilongjiang 161006)

Keywords:: single-cell clone; callus; esterase isozemes; peroxidase isozymes; enzyme spectrum

Abstract:: Taking single-cell clone Scutellaria baicalensis callus as material，using the polyacrylamide gel electrophoresis (PAGE) method，through the analysis of esterase isozyme (EST) and peroxidase isozyme (POD) enzyme band，Radix scutellariae singlecell clone callus and the genetic characteristics of callus and strain differences in parents were studied.The results showed that three bands were characteristics in EST and four bands were characteristics in POD in single-cell clone Scutellaria baicalensis callus of different strains，four bands were the most and three bands were the lest in EST in different strains，the first，the second and the third brands were the characteristic band；while the first，the third and the seventh brands were the characteristics bands in POD；differences were found in the presence or absence the number，width，color depth，the relative mobility of the same bands of EST and POD.There exist both genetic characteristics and strains difference in the single-cell clones Scutellaria callus and the parental cells，and these characteristics provide a theoretical reference in genetic characteristics and strains identification of the single-cell clone Scutellaria baicalensis (HQSC) callus.

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Last Update: 2015-07-16