|Table of Contents|

Study on Conditions of Pollen Culture in vitro and Growth Dynamic of Pollen Tube in Prunus sibirica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年07
Page:
81-84
Research Field:
Publishing date:

Info

Title:
Study on Conditions of Pollen Culture in vitro and Growth Dynamic of Pollen Tube in Prunus sibirica
Author(s):
LI Min; LIU Ming-guo; WU Yue-liang; DONG Sheng-jun
（College of Forestry，Shenyang Agricultural University，Shenyang，Liaoning 110866）
Keywords:
boric acid concentration; sampling method; pollen germination rate; pollen tube growth; Prunus sibirica
PACS:
-
DOI:
10.11937/bfyy.201507025
Abstract:
Taking excellent clones of Prunus sibirica as test material, using the method of pollen culture in vitro，the effect of in vitro culture on pollen of Prunus sibirica，different ways of sampling effect on pollen germination rate and the process of pollen tube growth were studied，in order to provide the scientific basis for understanding and controlling of the pollination and fertilization of the tree species. The results showed that, when the concentration of sucrose was 10.0% and the agar was 0.5%，the boric acid concentration of 0.010% was the most suitable for pollen culture in vitro. The pollen vitality of indoor hydroponic flower sampling was significantly lower than the pollen of outdoor natural flower sampling，which meant that in the relevant scientific research and production practice, try to shorten the indoor hydroponic time or obtain pollen from the natural blossom in field. Different Prunus sibirica clones had evidently different pollen viability.The growth process of pollen tubes in culture could be divided into four periods: pollen germination period (0.3-0.6 hours), rapid growth period (0.6-2.0 hours), slowing growth period (2.0-4.0 hours) and growth lag phase (after 4.0 hours).

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Last Update: 2015-07-16