|Table of Contents|

Construction of Fingerprint and Analysis of Genetic Diversity with EST-SSR Markers for Thirty-seven Peony Cultivars in Shandong(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年07
Page:
77-81
Research Field:
Publishing date:

Info

Title:
Construction of Fingerprint and Analysis of Genetic Diversity with EST-SSR Markers for Thirty-seven Peony Cultivars in Shandong
Author(s):
YANG Zhi-gang; ZHU Dong; LUO Bing; SUN Hai-yan
(Department of Bioengineering,Changshu Institute of Technology,Changshu,Jiangsu 215500)
Keywords:
EST-SSR; DNA fingerprint; genetic diversity; peony
PACS:
-
DOI:
10.11937/bfyy.201507024
Abstract:
Taking thirty-seven peony cultivars from Shandong as experimental materials,the DNA fingerprinting database was successfully constructed,and the genetic diversity was analyzed based on ten evenly distributed EST-SSR primer pairs with high polymorphisms and good repeatability.The results showed that,among the 37 varieties，10 primer pairs had 58 polymorphic locis，and 5.8 polymorphic locis were detected by each EST-SSR primer pair on an average with the range from 2 to 13.EST-SSR markers were suitable for using in construction of peony DNA fingerprint with polymorphism differences between the selected varieties.Thirty-seven cultivars could be identified by 4 primer combinations st least.The genetic deversity analyzed indicated that，37 peony varieties could be divided into five types based on the genetic similarity coefficient of 0.69 threshold.

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Last Update: 2015-07-16