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Research on Physiological and Biochemical Characters of Leaf Color Mutants in Chinese Ochid(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年07
Page:
65-68
Research Field:
Publishing date:

Info

Title:
Research on Physiological and Biochemical Characters of Leaf Color Mutants in Chinese Ochid
Author(s):
JIANG Yu1; HE Jun-rong1; XIONG Jian-rui1; 2; LI Ping2; ZHUO Bi-ping1
(1.Key Laboratory of Horticultural Crops，Biology and Germplasm Enhancement in Southwest Regions，Ministry of Agriculture，Institute of Horticulture，Sichuan Academy of Agricultural Sciences，Chengdu，Sichuan 610066；2.School of Life Science and Engineering，Southwest Jiaotong University，Chengdu，Sichuan 610031)
Keywords:
Chinese ochid; leaf color mutant; physiological and biochemical characters
PACS:
-
DOI:
10.11937/bfyy.201507021
Abstract:
The in vitro shoots of Cymbidium longibracteaturn‘Longchangsu’ with verge line pattern leaves and normal Cymbidium longibracteaturn‘Longchangsu’ as materials,the effect of multiplication and differentiation on the mutants were studied by the detection of the multiplication weight and differentiation rate and physiological and biochemical index determination of the leaves and rhizomes.The results showed that the rhizomes’ multiplication and differentiation were lower than those of the contrasts，so was the average height.The mutants’ chlorophyll a and chlorophyll b and chlorophyll a+b were 16.71%,15.86%,16.49% of the contrasts respectively.Chlorophyll b and carotenoids/chlorophyll were increased while chlorophyll a was decreased during the differentiation from the rhizomes to shoots.The contents of soluble sugar and protein were decreased.MDA content and the relative conductivity were increased.The activities of antioxidant enzymes，such as catalase (CAT) and superoxide dismutase (SOD) were lower while peroxidase (POD) activity were higher in the mutants than in the contrasts.The multiplication and differentiation of the mutants were closely related to the physiological characteristics.

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Last Update: 2015-07-16