Study on Selective Breeding of Mushroom Pleurotus ferulae(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: XU Bing; YAO Lu-ye; ZHU Jing; JI Hong; (College of Biology and Food Engineering，Changshu Institute of Technology，Changshu Jiangsu 215500)

Keywords:: Pleurotus ferulae; UV mutagenesis; monospore hybridization; growth rate; esterase isoenzyme electrophoresis

Abstract:: With ‘Bailing No.0’,‘Bailing No.1’,‘Bailing No.2’ monospore as test materials,cross breeding and UV mutation breeding technology were used to select breeding mushroom Preurotus ferulae.UV mutagenic treatment under the condition of the fatality rate of 75%.As parents，the single spore hybridization method was adopted to mononuclear mycelium after mating，Hybrids were primarily identified with clamp connection,then confirmed by antagonistic test and esterase isoenzyme analysis.The 26 strains were selected based on the comparison of mycelial growth rate，color and density among parent strain and hybrids.The results showed that Pleurotus ferulae spores treated by UV mutagenesis had high cross compatibility，and then hybrid had the characteristics of high positive mutation rate.

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Last Update: 2015-06-26