Isatis indigotica Fort.germplasm resources were very important for research.Genetic diversity study on Isatis indigotica Fort.was beneficial to the collection，management and utilization of Isatis indigotica Fort.of genetic resources.In order to make efficient use of Isatis indigotica Fort.germplasm resources，the phenophase and main agronomic traits for germplasm and materials of 32 Isatis indigotica Fort.were compared at seed beating period，under the open field cultivation on ditions.The experimental results showed that among the different lines，the widest range of returning green stage could be 22 d，that of the leaf-expansion period could be 22 d，that of the squaring period could be 24 d，that of the blooming period could be 24 d，that of the bearing pod period could be 15 d，that of the number of branches per plant could be 22 poor months，that of the height was 70 cm，that of the grain weight was 10.21 g，that of the seed yield per plant was 101.0 g，which the seed weight of BLG2012-22 was the largest of 14.93 g，and the rest of the germplasm between the sui generis.