Isolation　and　Fungicide　Screening of Garlic Dry Rot Disease(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: CHEN Long; WANG Yong; LI Xu-shuang; CHEN Dian; （School of Horticulture，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Northeast Region，Ministry of Agriculture，Northeast Agricultural University，Harbin，Heilongjiang 150030）

Keywords:: garlic dry rot; Fusariwn oxysporum Schlecht; virulence regression equation; root wilt stop

Abstract:: Pathogenic bacteria was used as test materials,that were seperated from the garlic dry rot samples collected from Shuangcheng，Datong，Gannan and Wuchang，etc.of Heilongjiang province with tissue separation.Then morphological identification and ITS sequence determination were conducted，and simultaneous lab screening of four types of fungicides was performed.The results showed that the pathogenic bacteria of garlic dry rot was Fusariwn oxysporum Schlecht；60% purely white carbendazim and root wilt stop presented the most obvious antibacterial effects on the Fusariwn oxysporum Schlecht that inducing garlic dry rot.These two fungicides were selected to prepare drug-containing medium with different concentration gradients，and bacteriostatic experiments were performed and virulence regression equation was formulated.In accordance with the calculation of virulence regression equation，the EC50 values of root wilt stop and 60% purely white carbendazim were 29.65 mg/L and 69.82 mg/L when they were exerting the preventive and therapeutic effects on Fusariwn oxysporum Schlecht.The EC50 values of the tested fungicides demonstrated that root wilt stop had a better inhibitory effect on Fusariwn oxysporum Schlecht.

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Last Update: 2015-06-26